Dabir Pankaj, Dabir Snehal, Siva Prasad B V, Reddy M V R
Department of Biochemistry and J B Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram 442 102, Wardha, Maharashtra, India.
Infect Genet Evol. 2006 Jul;6(4):287-91. doi: 10.1016/j.meegid.2005.08.005. Epub 2005 Sep 29.
Molecular characterization of Wuchereria bancrofti is essential to develop suitable anti-filarial drugs and vaccines. We describe here isolation, sequence analysis and cloning of a partial cDNA of an enzyme superoxide dismutase from this parasite. The immunoscreening of a lambda zap W. bancrofti microfilarial (Mf) cDNA library with microfilaremic sera had resulted in the isolation of several seroreactive clones including, WbSOD. This clone contained a 309 bp insert and showed significant nucleotide and deduced amino acid sequence homologies to the superoxide dismutases of other nematode parasites. The antioxidant property of this enzyme may have important contribution in the defense mechanism of the parasite against host immune response.
班氏吴策线虫的分子特征分析对于开发合适的抗丝虫药物和疫苗至关重要。我们在此描述了从这种寄生虫中分离、序列分析和克隆一种超氧化物歧化酶部分cDNA的过程。用微丝蚴血症血清对λzap班氏吴策线虫微丝蚴（Mf）cDNA文库进行免疫筛选，结果分离出了几个血清反应性克隆，包括WbSOD。该克隆含有一个309 bp的插入片段，与其他线虫寄生虫的超氧化物歧化酶具有显著的核苷酸和推导氨基酸序列同源性。这种酶的抗氧化特性可能在寄生虫抵御宿主免疫反应的防御机制中发挥重要作用。
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