低分子量肝素对糖尿病大鼠早期肾病的保护作用

[The protective effect of low molecular weight heparin on early nephropathy in diabetic rats].

作者信息

Guo H, Gan L, Yu Z

机构信息

Renal Division, People's Hospital, Peking University, Beijing, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2001 Nov 10;81(21):1327-9.

PMID:16200729

Abstract

OBJECTIVE

To investigate the protective effect of low molecular heparin on early nephropathy in diabetic rats and to explore its possible mechanism.

METHODS

The rats were randomly divided into following groups: normal control rats, streptozotocin (STZ, 65 mg/kg)-induced diabetic rats and diabetic rats treated with low molecular weight heparin (Fraxiparine, Nadroparin calcium 205 AXaIU/kg). The activity of anti-Xa factor and the activated partial thromboplastin time (APIT) in plasma were assayed, the renal function and profile of kidney hypertrophy were measured, and the renal tissue were observed by light microscopy after 1, 2, 4 weeks of the treatment respectively.

RESULTS

Compared with the untreated diabetic group, except for higher kidney hypertrophy index, LMWH treated diabetic rats presented less urinary albumin excretion, lower creatinine clearance rate (Ccr) and longer APTT than diabetic group without any treatment. Meanwhile, renal pathologic changes in LMWH treated group were improved.

CONCLUSION

Low molecular weight heparin possesses a renal protective effect on early diabetic nephropathy and its possible mechanism may be associated with the beneficial effect of LMWH on regulating abnormal diabetic renal hemodynamics.

摘要

目的

探讨低分子肝素对糖尿病大鼠早期肾病的保护作用，并探讨其可能机制。

方法

将大鼠随机分为以下几组：正常对照大鼠、链脲佐菌素（STZ，65mg/kg）诱导的糖尿病大鼠以及用低分子肝素（速碧林，那屈肝素钙205AXaIU/kg）治疗的糖尿病大鼠。分别在治疗1、2、4周后检测血浆中抗Xa因子活性和活化部分凝血活酶时间（APTT），测定肾功能和肾脏肥大情况，并通过光学显微镜观察肾组织。