Safonova G D, Kovalenko A P
Morfologiia. 2005;127(2):44-7.
The object of this work was to study the morpho-functional state of spinal ganglion neurons and to conduct the comparative quantitative analysis of the changes of neuronglial relations after hindlimb elongation in mongrel dogs by 14-16% of its initial length using different elongation rates. The longitudinal 5 microm thick serial sections of L(VI), L(VII) and S(I) ganglia stained with Nissl's thionine and cresyl violet and Einarssons's gallocyanin-chrome alume were studied. By days 45-48 of an experiment the reversible changes in the structure of some part of neurons were demonstrated, which included cytoplasmic and nuclear hyperchromatism, peripheral chromatolysis, nuclear and nucleolar dislocation, increase in the number of peri- and interneuronal gliocytes. The changes were most marked in the ganglia ipsilateral to the lengthening side with distraction rate of 3 mm per day; they were minimal contralaterally with the lengthening rate of 1 mm per day.
本研究的目的是研究脊髓神经节神经元的形态功能状态,并通过不同的延长速率将杂种犬后肢延长至其初始长度的14-16%,对神经胶质关系变化进行比较定量分析。研究了用尼氏硫堇、甲酚紫和埃纳森氏没食子蓝铬明矾染色的L(VI)、L(VII)和S(I)神经节纵向5微米厚的连续切片。在实验的第45-48天,观察到神经元某些部分结构的可逆变化,包括细胞质和细胞核的深染、周边染色质溶解、细胞核和核仁移位、神经元周围和神经元间神经胶质细胞数量增加。在每天牵引速率为3毫米的延长侧同侧神经节中变化最为明显;在对侧,每天延长速率为1毫米时变化最小。