[接种表达登革热2型NS1基因的质粒可在小鼠中引发免疫反应]
[Inoculation of plasmid expressing the dengue 2 NS1 gene elicits immunity in mice].
作者信息
Yang Chun-yu, Chen Yuan, Yan Hui-jun, Fang Dan-yun, Xue Yao-hua, Jiang Li-fang
机构信息
Department of Microbiology, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar;19(1):71-3.
OBJECTIVE
To study cellular and humoral immune responses to NS1 protein in mice inoculated intramuscularly with recombinant plasmid expressing dengue 2 NS1 gene.
METHODS
The eukaryotic expressing plasmid pCNX2-NS1 was injected into tibialis anterior muscle in mice. The mice were subsequently boosted with the same dose and same method twice after the initial inoculation. The mice were killed at four-week intervals and their serum and spleen cells were harvested for further test.
RESULTS
Dengue 2 antibodies were detectable in the sera from inoculated animals four weeks after the last boost. The changes of CD4+ T lymphocyte and CD8+ T lymphocyte were also determined by flow cytometry.
CONCLUSION
The recombinant plasmid containing dengue 2 NS1 genes is immunogenic in intramuscularly inoculated mice. The vaccinated mice produced dengue-2 specific and long lasting immunity.
目的
研究肌肉注射表达登革2型NS1基因的重组质粒的小鼠对NS1蛋白的细胞免疫和体液免疫反应。
方法
将真核表达质粒pCNX2-NS1注射到小鼠胫前肌中。初次接种后，随后用相同剂量和相同方法对小鼠进行两次加强免疫。每隔四周处死小鼠，收集其血清和脾细胞进行进一步检测。
结果
末次加强免疫四周后，在接种动物的血清中可检测到登革2型抗体。还通过流式细胞术测定了CD4+T淋巴细胞和CD8+T淋巴细胞的变化。
结论
含有登革2型NS1基因的重组质粒在肌肉注射的小鼠中具有免疫原性。接种疫苗的小鼠产生了登革2型特异性和持久的免疫力。
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