Carsia Rocco V, John-Alder Henry B
Department of Cell Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJ 08084, USA.
Gen Comp Endocrinol. 2006 Jan 15;145(2):157-61. doi: 10.1016/j.ygcen.2005.08.007. Epub 2005 Oct 4.
Elucidation of the role of natriuretic peptides (NPs) in vertebrate adrenal steroidogenesis has been facilitated by the use of freshly dispersed adrenocortical cells. Our recent characterization of lizard adrenocortical cells [Carsia, R.V., John-Alder, H.B., 2003. Seasonal alterations in adrenocortical cell function associated with stress-responsiveness and sex in the Eastern Fence Lizard (Sceloporus undulatus). Horm. Behav. 43, 408-420] provided the opportunity to examine the influence of atrial natriuretic peptides (ANPs) and related NPs on reptilian adrenal steroidogenesis at the cellular level. In the present report, the action of NPs on lizard adrenal steroidogenesis was investigated using freshly dispersed adrenocortical cells derived from the Eastern Fence Lizard (Sceloporus undulatus). Basal production rates of aldosterone and corticosterone and maximal angiotensin II (ANG II)-induced production rates of these corticosteroids were inhibited with high efficacy (75-90%) by rat ANP at potencies of 0.4-0.7 nM. By contrast, rat ANP had no effect on maximal production rates of these corticosteroids in response to a maximal steroidogenic concentration of adrenocorticotropin (ACTH; 1 nM). However, rat ANP inhibited aldosterone and corticosterone production rates in response to a half-maximal steroidogenic concentration of ACTH (10 pM; approximately 50 pg/ml), albeit with less efficacy ( approximately 50%) and potency (approximately 6 nM) than for ANG II. Rat and eel ANP and rat and chicken brain natriuretic peptide (BNP) were equally efficacious at inhibiting maximal ANG II-induced aldosterone and corticosterone production but with different potencies. The order of inhibitory potency was rat ANP = chicken BNP > eel ANP > rat BNP. However, a specific peptide ligand for the NP clearance receptor was without effect. This study indicates that ANP and related NPs are efficacious inhibitors of lizard adrenal steroidogenesis by acting directly at the level of the adrenocortical cell.
使用新鲜分离的肾上腺皮质细胞有助于阐明利钠肽（NP）在脊椎动物肾上腺类固醇生成中的作用。我们最近对蜥蜴肾上腺皮质细胞的特征描述[卡尔西亚，R.V.，约翰 - 奥尔德，H.B.，2003年。东部围栏蜥蜴（Sceloporus undulatus）中与应激反应性和性别相关的肾上腺皮质细胞功能的季节性变化。激素行为。43，408 - 420]提供了在细胞水平上研究心房利钠肽（ANP）和相关NP对爬行动物肾上腺类固醇生成影响的机会。在本报告中，使用来自东部围栏蜥蜴（Sceloporus undulatus）的新鲜分离的肾上腺皮质细胞研究了NP对蜥蜴肾上腺类固醇生成的作用。大鼠ANP在0.4 - 0.7 nM的效力下，对醛固酮和皮质酮的基础产生率以及最大血管紧张素II（ANG II）诱导的这些皮质类固醇的产生率具有高效抑制作用（75 - 90%）。相比之下，大鼠ANP对这些皮质类固醇在最大促肾上腺皮质激素（ACTH；1 nM）的类固醇生成浓度下的最大产生率没有影响。然而，大鼠ANP抑制了醛固酮和皮质酮的产生率，以响应ACTH的半最大类固醇生成浓度（10 pM；约50 pg/ml），尽管其效力（约50%）和效能（约6 nM）低于ANG II。大鼠和鳗鱼ANP以及大鼠和鸡脑利钠肽（BNP）在抑制最大ANG II诱导的醛固酮和皮质酮产生方面同样有效，但效力不同。抑制效力顺序为大鼠ANP = 鸡BNP > 鳗鱼ANP > 大鼠BNP。然而，NP清除受体的特异性肽配体没有作用。这项研究表明，ANP和相关NP通过直接作用于肾上腺皮质细胞水平，是蜥蜴肾上腺类固醇生成的有效抑制剂。
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