HP1对哺乳动物体内着丝粒周围异染色质的表观遗传调控

Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1.

作者信息

Kourmouli Niki, Sun Yuh-man, van der Sar Sjaak, Singh Prim B, Brown Jeremy P

机构信息

Division of Tumor Biology, Department of Immunology and Cell Biology, Forschungszentrum Borstel, D-23845 Borstel, Germany.

出版信息

Biochem Biophys Res Commun. 2005 Nov 25;337(3):901-7. doi: 10.1016/j.bbrc.2005.09.132. Epub 2005 Sep 30.

DOI:10.1016/j.bbrc.2005.09.132

PMID:16213461

Abstract

We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it".

摘要

我们开发了一种模型系统，通过该系统可将HP1靶向缺乏Suv(3)9h1/2组蛋白甲基转移酶（HMTase）活性的胚胎干细胞中的着丝粒周围异染色质。如此靶向的HP1可在着丝粒周围异染色质处重建组蛋白H3的三甲基化赖氨酸9（Me(3)K9H3）和组蛋白H4的三甲基化赖氨酸20（Me(3)K20H4），这表明HP1可在体内调节这些组蛋白修饰的分布。HP1同型和异型异构体之间的相互作用在体内得到了证实，HP1与ESET/SETDB1 HMTase和ATRX染色质重塑酶之间的相互作用也得到了证实。我们得出结论，HP1不仅能“解读”组蛋白密码，还能“编写”组蛋白密码。