Ming Feng, Mi Guo Hua, Lu Qun, Yin Sai, Zhang Shan Shan, Guo Bin, Shen Da Leng
Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University 220 Handan Road, Shanghai 200433, PR China.
Cell Mol Biol Lett. 2005;10(3):401-11.
A putative high-affinity phosphate (Pi) transporter gene in rice (Oryza sativa), OsLPT1, was isolated by RT-PCR from the leaves of the plants. The 1635-bp nucleotide sequence of OsLPT1 spans an open reading frame encoding a polypeptide of 535 amino acids with sequence similarity to phosphate transporters from other plant species. Southern blot analysis showed that the OsLPT1 gene might be present in three transcripts in the rice genome. RT-PCR analysis demonstrated the expression of OsLPT1 in both leaves and roots. The expression of OsLPT1 in the roots was enhanced by Pi deprivation. In situ hybridization revealed OsLPT1 expression in mesophyll cells, xylem parenchyma and phloem cells in the leaves, and in the epidermis, exodermis, and in the vasculature surrounding metaxylem vessels in the roots. The data suggests that the OsLPT1 protein may be involved in enhancing phosphate uptake under conditions of Pi starvation, and in the translocation of Pi among cells in shoots to increase the efficiency of internal Pi use.
通过RT-PCR从水稻（Oryza sativa）植株叶片中分离出一个推测的高亲和力磷酸盐（Pi）转运蛋白基因OsLPT1。OsLPT1的1635个碱基对核苷酸序列跨越一个开放阅读框，编码一个由535个氨基酸组成的多肽，该多肽与其他植物物种的磷酸盐转运蛋白具有序列相似性。Southern杂交分析表明，OsLPT1基因在水稻基因组中可能以三种转录本形式存在。RT-PCR分析证明OsLPT1在叶片和根中均有表达。Pi缺乏会增强OsLPT1在根中的表达。原位杂交显示，OsLPT1在叶片的叶肉细胞、木质部薄壁细胞和韧皮部细胞中表达，在根的表皮、外皮层以及后生木质部导管周围的维管组织中也有表达。数据表明，OsLPT1蛋白可能参与在Pi饥饿条件下增强磷酸盐吸收，以及参与地上部细胞间Pi的转运以提高内部Pi利用效率。
