热休克蛋白70在人肝癌细胞系BEL-7402中陪伴甲胎蛋白。
Heat shock protein 70 chaperoned alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402.
作者信息
Wang Xiao-Ping, Wang Qiao-Xia, Li Hai-Yan, Chen Rui-Fen
机构信息
Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China.
出版信息
World J Gastroenterol. 2005 Sep 21;11(35):5561-4. doi: 10.3748/wjg.v11.i35.5561.
AIM
To investigate the interaction between heat shock protein 70 (HSP70) and ?-fetoprotein (AFP) in human hepatocellular carcinoma (HCC) cell line BEL-7402.
METHODS
The expression and localization of HSP70 and AFP in human HCC cell line BEL-7402 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. The interaction between HSP70 and AFP in HCC cells was analyzed by immunoprecipitation and Western blot.
RESULTS
Immunocytochemical staining detection showed that HCC cell BEL-7402 expressed a high level of HSP70 and AFP synchronously. Both were stained in cell plasma. AFP existed in the immunoprecipitate of anti-HSP70 mAb, while there was HSP70 in the immunoprecipitate of anti-AFP mAb.
CONCLUSION
HSP70 chaperones AFP in human HCC cell BEL-7402. The interaction between HSP70 and AFP in human HCC cell can be a new route to study the pathogenesis and immunotherapy of HCC.
目的
研究热休克蛋白70（HSP70）与甲胎蛋白（AFP）在人肝癌细胞系BEL - 7402中的相互作用。
方法
采用免疫细胞化学和间接免疫荧光细胞化学染色法检测人肝癌细胞系BEL - 7402中HSP70和AFP的表达及定位。通过免疫沉淀和蛋白质印迹法分析肝癌细胞中HSP70与AFP的相互作用。
结果
免疫细胞化学染色检测显示，肝癌细胞BEL - 7402同步高表达HSP70和AFP。两者均在细胞质中染色。AFP存在于抗HSP70单克隆抗体的免疫沉淀物中，而抗AFP单克隆抗体的免疫沉淀物中有HSP70。
结论
在人肝癌细胞BEL - 7402中，HSP70作为分子伴侣与AFP相互作用。人肝癌细胞中HSP70与AFP的相互作用可能是研究肝癌发病机制和免疫治疗的新途径。
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