静压对中国仓鼠卵巢细胞产生人粒细胞巨噬细胞刺激因子（hGM-CSF）的影响。
Effect of static pressure on human granulocyte-macrophage stimulating factor (hGM-CSF) production by Chinese hamster ovary cells.
作者信息
Gong Heng, Takagi Mutsumi, Moriyama Tetsuhiro, Ohno Tsuyoshi, Yoshida Toshiomi
机构信息
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
出版信息
J Biosci Bioeng. 2002;94(3):271-4. doi: 10.1263/jbb.94.271.
The effect of static pressure on the production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. Although constant static pressures between 0.15 and 0.9 MPa had little influence on specific growth rate, the specific production rate of hGM-CSF increased from 1.23 to 1.62 x 10(-13) mg/cell/h in proportion to static pressure. The specific rate of tPA production by the human embryo lung cell line MRC-5 also increased from 2.92 to 6.38 x 10(-12) mg/cell/h as static pressure increased. The expression level of hGM-CSF mRNA increased with the pressurization even for 4 h.
研究了静压对中国仓鼠卵巢（CHO）细胞系DR1000L4N产生人粒细胞巨噬细胞集落刺激因子（hGM-CSF）的影响。尽管0.15至0.9MPa之间的恒定静压对比生长速率影响很小，但hGM-CSF的比生产率与静压成比例地从1.23增加到1.62×10（-13）mg/细胞/小时。随着静压增加，人胚肺细胞系MRC-5产生tPA的比速率也从2.92增加到6.38×10（-12）mg/细胞/小时。即使加压4小时，hGM-CSF mRNA的表达水平也随加压而增加。
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