高山被孢霉1S-4突变体中Δ5去饱和酶基因突变位点的鉴定

Identification of mutation sites on delta5 desaturase genes from Mortierella alpina 1S-4 mutants.

作者信息

Abe Takahiro, Sakuradani Eiji, Ueda Takuji, Shimizu Sakayu

机构信息

Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.

出版信息

J Biosci Bioeng. 2005 Mar;99(3):296-9. doi: 10.1263/jbb.99.296.

DOI:10.1263/jbb.99.296

PMID:16233793

Abstract

The mutation sites on delta5 desaturase genes in delta5 desaturase-defective mutants derived from arachidonic acid-producing Mortierella alpina 1S-4 were identified. The mutations resulted in an amino acid replacement (G189E or W301Stop) and uncorrected transcription caused by recognition of an AG-terminal newly created on C207A gene mutation, resulting in low or no delta5 desaturase activity in these mutants.

摘要

鉴定了源自产花生四烯酸的高山被孢霉1S-4的Δ5去饱和酶缺陷型突变体中Δ5去饱和酶基因上的突变位点。这些突变导致氨基酸替换（G189E或W301Stop），并且由于识别C207A基因突变上新产生的AG末端而导致转录未校正，从而使这些突变体中的Δ5去饱和酶活性低或无活性。