通过可逆且可重复使用的C端硫醇手柄进行肽连接。

Peptide ligation by a reversible and reusable C-terminal thiol handle.

作者信息

Lu Yi-An, Tam James P

机构信息

Department of Biomedical Research, Scripps Research Institute, Jupiter, Florida 33458, USA.

出版信息

Org Lett. 2005 Oct 27;7(22):5003-6. doi: 10.1021/ol052000f.

DOI:10.1021/ol052000f

PMID:16235943

Abstract

[reaction: see text] Peptide ligation of noncysteinyl residues can be achieved conveniently by a reversible C-terminal thiol handle together with a Ag(+) ion-assisted S,N-acyl migration. The regenerated C-terminal handle permits tandem ligation of multiple segment.

摘要

[反应：见正文] 非半胱氨酰残基的肽连接可通过可逆的C端硫醇手柄以及Ag(+)离子辅助的S,N-酰基迁移方便地实现。再生的C端手柄允许多个片段的串联连接。