在PGF2α处理后的固定时间或自然发情时对奶牛进行低精液剂量子宫角内输精。
Low semen dose intracornual insemination of cows at fixed time after PGF2alpha treatment or at spontaneous estrus.
作者信息
Kurykin J, Jaakma U, Waldmann A, Jalakas M, Aidnik M, Majas L, Padrik P
机构信息
Department of Reproductive Biology, Institute of Veterinary Medicine and Animal Science, Estonian Agricultural University, 46 Kreutzwaldi St., 51006 Tartu, Estonia.
出版信息
Anim Reprod Sci. 2006 Sep;95(1-2):116-24. doi: 10.1016/j.anireprosci.2005.09.011. Epub 2005 Oct 19.
The numbers of spermatozoa per insemination and the site of semen deposition in the uterine horn appear to interact to influence pregnancy rate. In two experiments, the effect of a single low dose (2 x 10(6) spermatozoa) intracornual insemination (LD-ICI) on bovine pregnancy rate was compared with that of intracornual (SD-ICI) and conventional (SD-AI) inseminations of 40 x 10(6) spermatozoa. In Experiment 1, 157 cows were treated twice with PGF(2)alpha at a 14-day interval and inseminated at a fixed time (80-82 h) after the second PGF(2)alpha injection using LD-ICI (n=44), SD-ICI (n=61) or SD-AI (n=52). In LD-ICI and SD-ICI groups, semen was deposited in the horn ipsilateral to the ovulatory follicle close to the utero-tubal junction (LD-ICI-UTJ, n=33 and SD-ICI-UTJ, n=41) or in the middle part of the horn (LD-ICI-MH, n=11 and SD-ICI-MH, n=20). Pregnancy rates after LD-ICI-UTJ, LD-ICI-MH, SD-ICI-UTJ and SD-ICI-MH were 27%, 27%, 39% and 35%, respectively (P>0.05). The total pregnancy rate after LD-ICI (27%) did not differ (P>0.05) from that after SD-ICI (37%) or SD-AI (34%). In Experiment 2 (field trial), 362 cows were allotted, at spontaneous estrus, to LD-ICI-UTJ (n=86), LD-ICI-MH (n=97) or SD-AI (n=179). Pregnancy rates after LD-ICI and SD-AI were 47% and 45%, respectively (P>0.05). After LD-ICI-UTJ, the pregnancy rate (54%) did not differ significantly (P>0.05) to that obtained after LD-ICI-MH (41%) and after SD-AI (45%). The results of the study show that the single intracornual insemination of cows with 2 x 10(6) spermatozoa at fixed time, 80-82 h after the second PGF(2)alpha injection or at spontaneous estrus resulted in similar pregnancy percentage as intracornual and conventional inseminations with 40 x 10(6) spermatozoa per semen dose. With intracornual insemination using low or standard dose of spermatozoa, the pregnancy rates were not significantly affected by the exact site of semen deposition in the uterine horn, near the utero-tubal junction or in the middle part.
每次输精的精子数量以及精液在子宫角内的沉积部位似乎相互作用，影响着妊娠率。在两项实验中，比较了单次低剂量（2×10⁶个精子）子宫角内输精（LD - ICI）对牛妊娠率的影响与40×10⁶个精子的子宫角内输精（SD - ICI）和常规输精（SD - AI）的效果。在实验1中，157头母牛每隔14天用PGF₂α处理两次，并在第二次PGF₂α注射后的固定时间（80 - 82小时）进行输精，使用LD - ICI（n = 44）、SD - ICI（n = 61）或SD - AI（n = 52）。在LD - ICI和SD - ICI组中，精液沉积在靠近子宫输卵管交界处的排卵卵泡同侧子宫角（LD - ICI - UTJ，n = 33和SD - ICI - UTJ，n = 41）或子宫角中部（LD - ICI - MH，n = 11和SD - ICI - MH，n = 20）。LD - ICI - UTJ、LD - ICI - MH、SD - ICI - UTJ和SD - ICI - MH后的妊娠率分别为27%、27%、39%和35%（P>0.05）。LD - ICI后的总妊娠率（27%）与SD - ICI（37%）或SD - AI（34%）后的总妊娠率无差异（P>0.05）。在实验2（田间试验）中，362头母牛在自然发情时被分配到LD - ICI - UTJ（n = 86）、LD - ICI - MH（n = 97）或SD - AI（n = 179）组。LD - ICI和SD - AI后的妊娠率分别为47%和45%（P>0.05）。LD - ICI - UTJ后的妊娠率（
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