环糊精修饰胶束电动色谱法分离共轭亚油酸异构体

Separation of conjugated linoleic acid isomers by cyclodextrin-modified micellar electrokinetic chromatography.

作者信息

Liu Xiaohua, Cao Yusheng, Chen Yan

机构信息

Sino-German Joint Research Institute, Nanchang University, China.

出版信息

J Chromatogr A. 2005 Nov 18;1095(1-2):197-200. doi: 10.1016/j.chroma.2005.09.090. Epub 2005 Oct 21.

DOI:10.1016/j.chroma.2005.09.090

PMID:16243343

Abstract

A cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) method was developed for separating conjugated linoleic acid (CLA) isomers. All the seven CLA isomers (9cis,11cis-CLA, 9cis,11trans-CLA, 9trans,11trans-CLA, 10trans,12cis-CLA, 11cis,13cis-CLA, 11cis,13trans-CLA and 11trans,13trans-CLA) were completely separated in the optimized conditions (4% (w/v) beta-cyclodextrin (beta-CD), 54 mM sodium dodecyl sulphate (SDS), 80 mM borate (pH 9.0), 8 M urea, 4% (v/v) ethanol, 30 kV and 15 degrees C). The CD-MEKC method was superior to the gas chromatographic (GC) and silver-ion high-performance liquid chromatographic (Ag(+)-HPLC) methods that were generally used in analyzing CLA isomers.

摘要

建立了一种环糊精修饰的胶束电动色谱法（CD-MEKC）用于分离共轭亚油酸（CLA）异构体。在优化条件下（4%（w/v）β-环糊精（β-CD）、54 mM十二烷基硫酸钠（SDS）、80 mM硼酸盐（pH 9.0）、8 M尿素、4%（v/v）乙醇、30 kV和15℃），所有七种CLA异构体（9顺式，11顺式-CLA、9顺式，11反式-CLA、9反式，11反式-CLA、10反式，12顺式-CLA、11顺式，13顺式-CLA、11顺式，13反式-CLA和11反式，13反式-CLA）都能完全分离。CD-MEKC方法优于通常用于分析CLA异构体的气相色谱（GC）法和银离子高效液相色谱（Ag(+)-HPLC）法。