克隆并鉴定一种新型人类1-酰基-sn-甘油-3-磷酸酰基转移酶基因AGPAT7。
Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7.
作者信息
Ye Guang-Ming, Chen Cong, Huang Song, Han Ding-Ding, Guo Jin-Hu, Wan Bo, Yu Long
机构信息
School of Life Sciences, Fudan University, State Key Laboratory of Genetic Engineering, Shanghai, 200433, P R China.
出版信息
DNA Seq. 2005 Oct;16(5):386-90. doi: 10.1080/10425170500213712.
The 1-Acylglycerolphosphate acyltransferase is crucial enzyme for synthesis of glycerolipids as well as triacylglylcerol biosynthesis in eukaryotes. Six members of 1-acyl-sn-glycerol-3-phosphate acyltransferase family in human have been described, which were AGPAT1, 2, 3, 4, 5 and 6. Here we report the cloning and characterization of another novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase member AGPAT7 (1-acyl-sn-glycerol-3-phosphate acyltransferase 7) gene, which was mapped to human chromosome 15q14. The AGPAT7 cDNA is 1898 bp in length, encoding a putative protein with 524 amino acid residues, which contains an acyltransferase domain in 123-234 aa. RT PCR amplification in 18 human tissues indicated that human AGPAT7 gene was widely expressed in uterus, thymus, pancreas, skeletal muscle, bladder, stomach, lung and testis. AGPAT7 protein was mainly localized to the endoplasmic reticulum (ER) in Hela cells.
1-酰基甘油磷酸酰基转移酶是真核生物中甘油脂质合成以及三酰甘油生物合成的关键酶。人类1-酰基-sn-甘油-3-磷酸酰基转移酶家族的六个成员已被描述，分别为AGPAT1、2、3、4、5和6。在此，我们报告另一个新的人类1-酰基-sn-甘油-3-磷酸酰基转移酶成员AGPAT7（1-酰基-sn-甘油-3-磷酸酰基转移酶7）基因的克隆和特征，该基因定位于人类染色体15q14。AGPAT7 cDNA长度为1898 bp，编码一个含有524个氨基酸残基的假定蛋白质，该蛋白质在123 - 234氨基酸处含有一个酰基转移酶结构域。在18种人类组织中的RT PCR扩增表明，人类AGPAT7基因在子宫、胸腺、胰腺、骨骼肌、膀胱、胃、肺和睾丸中广泛表达。AGPAT7蛋白主要定位于Hela细胞的内质网（ER）。
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