Jiang De-Sheng, Long Sheng-Ya, Huang Jun, Xiao Hai-Yan, Zhou Ju-ying
Key Laboratory of Fiber Optic Sensing Technology and Information Processing, Ministry of Education, Fiber Optic Sensing Technology Research Center, Wuhan University of Technology, Wuhan 430070, China.
Wei Sheng Wu Xue Bao. 2005 Aug;45(4):630-3.
Laccase was immobilized on magnetic chitosan microspheres by using glutaraldehyde as cross-linking reagent. The immobilization conditions and characterization of the immobilized enzyme were investigated. immobilization conditions for laccase were: 10mL of 0.8mg/mL of laccase in phosphate buffer(0.1mol/L, pH 7.0) reacted with 50mg of magnetic chitosan microspheres at 25 degrees C for 1h and subsequently was kept at 4 degrees C for 2h. The immobilized enzyme exhibited the maximal activity at pH 3.0. The optimal temperatures for immobilized enzyme were 10 degrees C and 55 degrees C. The Km value of immobilized laccase for ABTS was 171.1 micromol/L in pH 3.0 phosphate buffer at 37 degrees C. Compared with free enzyme, the thermal, operational, and storage stabilities of the enzyme were increased after the immobilization.
以戊二醛为交联剂，将漆酶固定在磁性壳聚糖微球上。研究了固定化条件及固定化酶的表征。漆酶的固定化条件为：在25℃下，10mL 0.8mg/mL的漆酶在磷酸盐缓冲液（0.1mol/L，pH 7.0）中与50mg磁性壳聚糖微球反应1h，随后在4℃下保存2h。固定化酶在pH 3.0时表现出最大活性。固定化酶的最适温度为10℃和55℃。在37℃、pH 3.0的磷酸盐缓冲液中，固定化漆酶对ABTS的Km值为171.1μmol/L。与游离酶相比，固定化后酶的热稳定性、操作稳定性和储存稳定性均有所提高。
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