Matsushita S, Yamada S, Kudoh Y
Department of Microbiology, Tokyo Metropolitan Research Laboratory of Public Health.
Kansenshogaku Zasshi. 1992 Apr;66(4):503-7. doi: 10.11150/kansenshogakuzasshi1970.66.503.
Five Shigella strains isolated from stool cultures of two domestic dysentery cases and three imported cases in Tokyo in 1986-1989 had an atypial serotype that agglutinated with only antiserum to S. flexneri group 6 factor antigen. These strains showed typical biochemical reactions of S. flexneri and were biochemically identical. Four of the strains were positive for Serény test with guinea pig eye and the cell-invasion test with HeLa cells. The strains also had the virulence-plasmid encoding outer membrane proteins, indicating that these were pathogenic. These strains were serologically identical and gave significant cross-reactions with S. flexneri 1b and 4b that had group 6 factor antigen. However, results of reciprocal absorption tests showed that the O antigen of these strains were not identical to S. flexneri 1b and 4b, and were confirmed to have a new type antigen which can not be included among the known S. flexneri type antigen I-VI. Strain 88-893 is designated as the test strain for this new type antigen of S. flexneri.
1986年至1989年期间，从东京两例国内痢疾病例和三例输入性病例的粪便培养物中分离出的5株志贺氏菌具有非典型血清型，仅与弗氏志贺氏菌6群因子抗原的抗血清发生凝集反应。这些菌株表现出弗氏志贺氏菌典型的生化反应，且生化特性相同。其中4株对豚鼠眼的Serény试验和对HeLa细胞的细胞侵袭试验呈阳性。这些菌株还具有编码外膜蛋白的毒力质粒，表明它们具有致病性。这些菌株血清学特性相同，与具有6群因子抗原的弗氏志贺氏菌1b和4b有明显的交叉反应。然而，双向吸收试验结果表明，这些菌株的O抗原与弗氏志贺氏菌1b和4b不同，证实具有一种新型抗原，不能归入已知的弗氏志贺氏菌I-VI型抗原中。菌株88-893被指定为这种新型弗氏志贺氏菌抗原的试验菌株。
