炎症性肠病中的抗中性粒细胞胞浆自身抗体和抗酿酒酵母抗体。

Antineutrophil cytoplasmic autoantibodies and anti-Saccharomyces cerevisiae antibodies in inflammatory bowel diseases.

作者信息

Kiliç Z Mesut Yalin, Tunç Bilge, Ayaz Selime, Filik Levent, Aktaş Semra, Parlak Erkan, Ulker Aysel

机构信息

Gastroenterology Clinic, Türkiye Yüksek Ihtisas Hospital, Ankara, Turkey.

出版信息

Turk J Gastroenterol. 2004 Dec;15(4):238-42.

PMID:16249978

Abstract

BACKGROUND/AIMS: Perinuclear antineutrophil cytoplasmic autoantibody is a marker for ulcerative colitis, and anti-Saccharomyces cerevisiae antibody is known to be associated with Crohn's disease. The purpose of this study was to search the value of detecting perinuclear antineutrophil cytoplasmic autoantibody and anti-Saccharomyces cerevisiae antibody for the diagnosis of Turkish inflammatory bowel disease patients.

METHODS

Serum samples were obtained from 80 patients with ulcerative colitis, 61 patients with Crohn's disease and 40 healthy controls. Determination of both anti-Saccharomyces cerevisiae antibody and antineutrophil cytoplasmic autoantibody was performed with the standardized enzyme-linked immunosorbent assay.

RESULTS

In cases with ulcerative colitis, 65% tested seropositive for antineutrophil cytoplasmic autoantibody, whereas the controls showed 2.5% positivity. In cases with Crohn's disease, 63.9% tested seropositive for anti-Saccharomyces cerevisiae antibody, whereas the controls showed 2.5% seropositivity. The combination of a positive anti-Saccharomyces cerevisiae antibody test and a negative antineutrophil cytoplasmic autoantibody yielded a sensitivity and specificity of 32.0% and 97.5%, respectively. The combination of a positive perinuclear antineutrophil cytoplasmic autoantibody and a negative anti-Saccharomyces cerevisiae antibody test yielded a sensitivity and specificity of 44.2% and 97.5%, respectively.

CONCLUSIONS

Both serologic tests may aid in the differential diagnosis of inflammatory bowel disease.

摘要

背景/目的：核周型抗中性粒细胞胞浆抗体是溃疡性结肠炎的一个标志物，而抗酿酒酵母抗体已知与克罗恩病相关。本研究的目的是探寻检测核周型抗中性粒细胞胞浆抗体和抗酿酒酵母抗体对诊断土耳其炎性肠病患者的价值。

方法

从80例溃疡性结肠炎患者、61例克罗恩病患者及40例健康对照者中获取血清样本。采用标准化酶联免疫吸附测定法检测抗酿酒酵母抗体和抗中性粒细胞胞浆抗体。

结果

在溃疡性结肠炎患者中，65%的患者抗中性粒细胞胞浆抗体检测呈血清阳性，而对照组的阳性率为2.5%。在克罗恩病患者中，63.9%的患者抗酿酒酵母抗体检测呈血清阳性，而对照组的血清阳性率为2.5%。抗酿酒酵母抗体检测阳性且抗中性粒细胞胞浆抗体检测阴性的联合检测，其敏感性和特异性分别为32.0%和97.5%。核周型抗中性粒细胞胞浆抗体检测阳性且抗酿酒酵母抗体检测阴性的联合检测，其敏感性和特异性分别为44.2%和97.5%。