来自人类白内障晶状体的谷胱甘肽还原酶可通过还原剂和分子伴侣α-晶状体蛋白恢复活性。
Glutathione reductase from human cataract lenses can be revived by reducing agents and by a molecular chaperone, alpha-crystallin.
作者信息
Rachdan Diyaa, Lou Marjorie F, Harding John J
机构信息
Nuffield Laboratory of Ophthalmology, University of Oxford, UK.
出版信息
Curr Eye Res. 2005 Oct;30(10):919-25. doi: 10.1080/02713680590953110.
PURPOSE
The aim of this study was to investigate how glutathione reductase (GR) loses its activity during cataract formation and whether it is possible to revive it back to the normal levels.
METHOD
In this study, endogenous as well as synthetic reducing systems (GSH, TTase, DTT, captopril) and alpha-crystallin at different concentrations were incubated with the soluble fraction of human cataract lens protein. The activity of glutathione reductase with or without the reducing agents and alpha-crystallin was tested, and the difference in activity gained was calculated.
RESULTS
Five agents (GSH, DTT, TTase, captopril, alpha-low crystallin) were able to revive the activity of GR from human cataract lenses to different extents.
CONCLUSION
This study shows that human lens GR activity was revived by different reducing agents as well as by a molecular chaperone (alpha-crystallin).
目的
本研究旨在探讨谷胱甘肽还原酶（GR）在白内障形成过程中如何丧失活性，以及是否有可能将其恢复到正常水平。
方法
在本研究中，将内源性以及合成还原系统（谷胱甘肽、转硫酶、二硫苏糖醇、卡托普利）和不同浓度的α-晶状体蛋白与人白内障晶状体蛋白的可溶性部分一起孵育。测试了添加或不添加还原剂及α-晶状体蛋白时谷胱甘肽还原酶的活性，并计算获得的活性差异。
结果
五种试剂（谷胱甘肽、二硫苏糖醇、转硫酶、卡托普利、低浓度α-晶状体蛋白）能够不同程度地恢复人白内障晶状体中GR的活性。
结论
本研究表明，不同的还原剂以及分子伴侣（α-晶状体蛋白）可恢复人晶状体GR的活性。
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