Karahan Z C, Akar N
Division of Pediatric Molecular Pathology and Genetics, Ankara University Faculty of Medicine, 06100 Cebeci, Ankara, Turkey.
Microbiol Res. 2005;160(4):361-6. doi: 10.1016/j.micres.2005.02.010.
Systemic yeast infections are the Leading cause of mortality and morbidity in immunocompromized patients. Candida albicans, being the most frequently isolated fungal pathogen in these patients, can be divided into three genotypes (genotypes A, B and C) by 25S intron analysis. In our study, we found that molecular sizes of genotype A C. albicans isolates were heterogeneous. In order to determine the molecular basis of this difference, Haelll digestion was applied, and strains forming different band patterns were analyzed by automated sequence analysis. As a result of sequence analysis, eight different subtypes (a --> h) were found among genotype A C. albicans strains and an easy differentiation scheme consisting of Haelll and MspI digestions was constructed.
全身性酵母菌感染是免疫功能低下患者死亡和发病的主要原因。白色念珠菌是这些患者中最常分离出的真菌病原体，通过25S内含子分析可分为三种基因型（基因型A、B和C）。在我们的研究中，我们发现基因型A白色念珠菌分离株的分子大小是异质的。为了确定这种差异的分子基础，应用了HaeIII消化，并通过自动序列分析对形成不同条带模式的菌株进行了分析。序列分析的结果是，在基因型A白色念珠菌菌株中发现了八种不同的亚型（a→h），并构建了一种由HaeIII和MspI消化组成的简易鉴别方案。
