Silva-Stenico Maria Estela, Pacheco Flávia Tereza Hansen, Rodrigues Jorge Luiz Mazza, Carrilho Emanuel, Tsai Siu Mui
Laboratório de Biologia Celular e Molecular, Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil.
Microbiol Res. 2005;160(4):429-36. doi: 10.1016/j.micres.2005.03.007.
In this study, the production of siderophores by Xylella fastidiosa from the citrus bacteria isolate 31b9a5c (FAPESP - ONSA, Brazil) was investigated. The preliminary evidence supporting the existence of siderophore in X. fastidiosa was found during the evaluation of sequencing data generated in our lab using the BLAST-X tool, which indicated putative open reading frames (ORFs) associated with iron-binding proteins. In an iron-limited medium siderophores were detected in the supernatant of X. fastidiosa cultures. The endophytic bacterium Methylobacterium extorquens was also evaluated. Capillary electrophoresis was used to separate putative siderophores produced by X. fastidiosa. The bacterial culture supernatants of X. fastidiosa were identified negative for hydroxamate and catechol and positive for M. extorquens that secreted hydroxamate-type siderophores.
在本研究中，对来自柑橘细菌分离株31b9a5c（巴西圣保罗研究基金会 - ONSA）的桑氏木质部菌（Xylella fastidiosa）产生铁载体的情况进行了调查。在使用BLAST-X工具评估我们实验室生成的测序数据时，发现了支持桑氏木质部菌中存在铁载体的初步证据，该证据表明存在与铁结合蛋白相关的推定开放阅读框（ORF）。在铁限制培养基中，在桑氏木质部菌培养物的上清液中检测到了铁载体。还对内生细菌扭脱甲基杆菌（Methylobacterium extorquens）进行了评估。采用毛细管电泳法分离桑氏木质部菌产生的推定铁载体。桑氏木质部菌的细菌培养上清液经鉴定对异羟肟酸和儿茶酚呈阴性，而对分泌异羟肟酸型铁载体的扭脱甲基杆菌呈阳性。
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