[ECV304细胞表达的HLA - G1分子抑制同种异体自然杀伤细胞的细胞毒性活性]
[HLA-G1 molecule expressed by ECV304 cells inhibit cytotoxic activity of allogeneic NK cells].
作者信息
Zhang Cai-e, Han Jun-yan, Yang Hui-jun, Wu Xiong-wen, Huang Ya-fei, Liang Zhi-hui, Gong Fei-li
机构信息
Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Nov;21(6):676-8.
AIM
To study the effect of HLA-G1 molecule expressed by an endothelial cell line (ECV304) on the cytotoxic activity of allogeneic NK cells.
METHODS
ECV304 cells were transfected with recombinant plasmid pcDNA3-HLA-G1 by the liposome transfection, and the expressed HLA-G1 on the cell surface was detected by indirect immunofluorescent assay and flow cytometry. The cytotoxic activity of allogeneic NK cells against ECV304 cells was analyzed by the MTT method.
RESULTS
HLA-G1 was expressed on the surface of the transfected ECV304 cells. The specific lysis of NK cells against plasmid pcDNA3 transfected ECV304 was (50.6+/-18.1)%, while the specific lysis against pcDNA3-HLA-G1 transfected ECV304 was (29.7+/-11.4)%, which was significantly lower than the former (P<0.001).
CONCLUSION
HLA-G1 expressed by the ECV304 cells can inhibit cytotoxicity of allogeneic NK cells.
目的
研究内皮细胞系（ECV304）表达的HLA - G1分子对同种异体自然杀伤细胞（NK细胞）细胞毒活性的影响。
方法
采用脂质体转染法将重组质粒pcDNA3 - HLA - G1转染至ECV304细胞，通过间接免疫荧光检测和流式细胞术检测细胞表面表达的HLA - G1。采用MTT法分析同种异体NK细胞对ECV304细胞的细胞毒活性。
结果
转染后的ECV304细胞表面表达HLA - G1。NK细胞对转染质粒pcDNA3的ECV304细胞的特异性杀伤率为（50.6±18.1）%，而对转染pcDNA3 - HLA - G1的ECV304细胞的特异性杀伤率为（29.7±11.4）%，显著低于前者（P<0.001）。
结论
ECV304细胞表达的HLA - G1可抑制同种异体NK细胞的细胞毒性。
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