Chen Gang, Fu Zhi-ren, Wang Liang-hua, Jiao Bing-hua
Department of Hepatic and Biliary Surgery, Kunming General Hospital, Kunming 650032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Nov;21(6):775-7.
To investigate the effect of human interleukin-4 (hIL-4) on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells(BAEC) activated with TNF-alpha.
BAEC were cultured in different concentrations of hIL-4 for 2 h followed by treatment with 4 microg/L TNF-alpha for 6 h or 18 h. The expression of E-selectin and ICAM-1 on BAEC was detected by cell-ELISA. The effect of hIL-4 on viability of BAEC was detected by MTT colorimetry.
Pretreatment of BAEC with hIL-4 inhibited the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha in a dose-dependent manner (P<0.01). Both TNF-alpha and hIL-4 had no effect on the viability of BAEC.
hIL-4 can suppress the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha, which may contribute to the xenotransplant immune tolerance associated with hIL-4.
研究人白细胞介素-4（hIL-4）对经肿瘤坏死因子-α（TNF-α）激活的牛主动脉内皮细胞（BAEC）上E-选择素和细胞间黏附分子-1（ICAM-1）表达的影响。
将BAEC培养于不同浓度的hIL-4中2小时，随后用4μg/L TNF-α处理6小时或18小时。通过细胞酶联免疫吸附测定法检测BAEC上E-选择素和ICAM-1的表达。通过MTT比色法检测hIL-4对BAEC活力的影响。
用hIL-4预处理BAEC可剂量依赖性地抑制经TNF-α激活的BAEC上E-选择素和ICAM-1的表达（P<0.01）。TNF-α和hIL-4对BAEC的活力均无影响。
hIL-4可抑制经TNF-α激活的BAEC上E-选择素和ICAM-1的表达，这可能有助于与hIL-4相关的异种移植免疫耐受。
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