Xiang Tai-He, Wang Li-Lin, Pang Ji-Liang, Chen Min, Xu Chao
School of Life Sciences, Hangzhou Normal College, Hangzhou 310036, China.
Yi Chuan. 2005 Sep;27(5):783-6.
The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91%, respectively. Moreover, hairy root was induced from healthy cucumber axillary bud with frequency of 10%. PCR analysis of hairy root DNA was conducted using the primers from rolC gene. The PCR results showed that all hairy root lines contained T-DNA. The established system should be ideal for studying soybean and cucumber nematode and garden balsam breeding of flower dwarf architecture.
用野生型发根农杆菌K599对大豆、黄瓜和凤仙花的幼苗进行接种，在接种部位诱导出了毛状根。大豆、黄瓜和凤仙花伤口子叶的毛状根诱导频率分别为100%、65%和91%。此外，从健康黄瓜腋芽诱导出了毛状根，频率为10%。使用来自rolC基因的引物对毛状根DNA进行PCR分析。PCR结果表明，所有毛状根系均含有T-DNA。所建立的体系对于研究大豆和黄瓜线虫以及凤仙花花矮化结构的育种应该是理想的。
