Gu Xinbin, Schwartz Joel L, Pang Xiaowu, Zhou Yanfei, Sirois David A, Sridhar Rajagopalan
Department of Oral Diagnosis, College of Dentistry, Howard University, 600 W Street NW, Washington, DC 20059, USA.
Cancer Lett. 2006 Aug 8;239(2):281-91. doi: 10.1016/j.canlet.2005.08.028. Epub 2005 Nov 3.
There is compelling evidence for the cancer chemopreventive effects of vitamin E and related compounds. Of all the vitamin E derivatives that have been investigated to date, vitamin E acid succinate is the most effective anti-cancer agent. This report describes the preparation and testing of liposomal formulation of mono alpha-tocopheryl ester of succinic acid (alpha-TOS) for cytotoxicity against hamster cheek pouch carcinoma cell line (HCPC-1). Small unilamellar vesicles (SUV) of phosphatidylcholine incorporating 70 microM alpha-TOS were superior to alpha-TOS alone or SUV without incorporated alpha-TOS, as inducers of apoptosis in HCPC-1 cells. Liposomal alpha-TOS perturbed the lipid structure in cells, promoted apoptosis, and decreased cell viability. The mechanism of action of alpha-TOS appears to involve membrane damage and induction of ceramide mediated apoptosis.
有令人信服的证据表明维生素E及相关化合物具有癌症化学预防作用。在迄今为止研究的所有维生素E衍生物中,维生素E琥珀酸酯是最有效的抗癌剂。本报告描述了琥珀酸单α-生育酚酯(α-TOS)脂质体制剂对仓鼠颊囊癌细胞系(HCPC-1)细胞毒性的制备和测试。掺入70微摩尔α-TOS的磷脂酰胆碱小单层囊泡(SUV)作为HCPC-1细胞凋亡的诱导剂,优于单独的α-TOS或未掺入α-TOS的SUV。脂质体α-TOS扰乱细胞中的脂质结构,促进细胞凋亡,并降低细胞活力。α-TOS的作用机制似乎涉及膜损伤和神经酰胺介导的细胞凋亡诱导。