Borrego Emma, Proverbio Teresa, Marín Reinaldo, Proverbio Fulgencio
Laboratorio de Bioenergética Celular, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas 1020A, Venezuela.
Gynecol Obstet Invest. 2006;61(3):128-32. doi: 10.1159/000089491. Epub 2005 Nov 2.
The Ca-ATPase activity of the plasma membranes of several tissues of preeclamptic pregnant women is significantly reduced when compared with the values of normotensive pregnant women. This has been explained considering the raise in the level of lipid peroxidation of the plasma membranes with preeclampsia. In this work we studied the effect of lipid peroxidation of syncytiotrophoblast basal (fetal facing) plasma membranes from normotensive pregnant women, on their level of Ca-ATPase activity.
The syncytiotrophoblast basal (fetal facing) plasma membranes from normotensive pregnant women were isolated and irradiated with ultraviolet (UV) light (254 nm). The membranes were then assayed for Ca-ATPase activity and lipid peroxidation by TBARS.
The UV irradiation raises the level of lipid peroxidation of the membranes, producing a concomitant inhibition of their Ca-ATPase activity. Presence of the antioxidant butylated hydroxytoluene during the UV irradiation of the membranes prevents increase in their level of lipid peroxidation and hence the inhibition of their Ca-ATPase activity.
These results give a strong support to the hypothesis that the lowered Ca-ATPase activity already described for plasma membranes of several tissues of preeclamptic women is the consequence of the increased level of lipid peroxidation shown by these membranes.
与血压正常的孕妇相比，子痫前期孕妇多个组织的质膜中钙 - ATP酶活性显著降低。考虑到子痫前期时质膜脂质过氧化水平升高，这一现象得到了解释。在本研究中，我们探讨了血压正常孕妇的合体滋养层基底（面向胎儿）质膜脂质过氧化对其钙 - ATP酶活性水平的影响。
分离血压正常孕妇的合体滋养层基底（面向胎儿）质膜，并用紫外线（254nm）照射。然后通过硫代巴比妥酸反应物（TBARS）测定膜的钙 - ATP酶活性和脂质过氧化水平。
紫外线照射提高了膜的脂质过氧化水平，同时抑制了其钙 - ATP酶活性。在膜的紫外线照射过程中加入抗氧化剂丁基羟基甲苯可防止其脂质过氧化水平升高，从而避免其钙 - ATP酶活性受到抑制。
这些结果有力支持了以下假说：子痫前期孕妇多个组织质膜中已报道的钙 - ATP酶活性降低是这些膜脂质过氧化水平升高的结果。
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