细胞外三磷酸腺苷对食管癌细胞生长的抑制作用。
Inhibitory effects of extracellular adenosine triphosphate on growth of esophageal carcinoma cells.
作者信息
Wang Ming-Xia, Ren Lei-Ming, Shan Bao-En
机构信息
School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China.
出版信息
World J Gastroenterol. 2005 Oct 14;11(38):5915-9. doi: 10.3748/wjg.v11.i38.5915.
AIM
To study the growth inhibitory effects of ATP on TE-13 human squamous esophageal carcinoma cells in vitro.
METHODS
MTT assay was used to determine the inhibition of proliferation of ATP or adenosine (ADO) on TE-13 cell line. The morphological changes of TE-13 cells induced by ATP or ADO were observed under fluorescence light microscope by acridine orange (AO)/ethidium bromide (EB) double stained cells. The internucleosomal fragmentation of genomic DNA was detected by agarose gel electrophoresis. The apoptotic rate and cell cycle after treatment with ATP or ADO were determined by flow cytometry.
RESULTS
ATP and ADO produced inhibitory effects on TE-13 cells at the concentration between 0.01 and 1.0 mmol/L. The IC(50) of TE-13 cells exposed to ATP or ADO for 48 and 72 h was 0.71 or 1.05, and 0.21 or 0.19 mmol/L, respectively. The distribution of cell cycle phase and proliferation index (PI) value of TE-13 cells changed, when being exposed to ATP or ADO at the concentrations of 0.01, 0.1, and 1 mmol/L for 48 h. ATP and ADO inhibited the cell proliferation by changing the distribution of cell cycle phase via either G(0)/G(1) phase (ATP or ADO, 1 mmol/L) or S phase (ATP, 0.1 mmol/L) arrest. Under light microscope, the tumor cells exposed to 0.3 mmol/L ATP or ADO displayed morphological changes of apoptosis. A ladder-like pattern of DNA fragmentation was obtained from TE-13 cells treated with 0.1-1 mmol/L ATP or ADO in agarose gel electrophoresis. ATP and ADO induced apoptosis of TE-13 cells in a dose-dependent manner at the concentration between 0.03 and 1 mmol/L. The maximum apoptotic rate of TE-13 cells exposed to ATP or ADO for 48 h was 16.63% or 16.9%, respectively.
CONCLUSION
ATP and ADO inhibit cell proliferation, arrest cell cycle, and induce apoptosis of TE-13 cell line.
目的
研究三磷酸腺苷（ATP）对人食管鳞状癌细胞系TE - 13的体外生长抑制作用。
方法
采用MTT法检测ATP或腺苷（ADO）对TE - 13细胞系增殖的抑制作用。用吖啶橙（AO）/溴化乙锭（EB）双染细胞，在荧光显微镜下观察ATP或ADO诱导的TE - 13细胞形态变化。通过琼脂糖凝胶电泳检测基因组DNA的核小体间断裂。用流式细胞术检测ATP或ADO处理后细胞的凋亡率和细胞周期。
结果
ATP和ADO在0.01至1.0 mmol/L浓度范围内对TE - 13细胞产生抑制作用。TE - 13细胞暴露于ATP或ADO 48小时和72小时的半数抑制浓度（IC50）分别为0.71或1.05 mmol/L，以及0.21或0.19 mmol/L。当TE - 13细胞在0.01、0.1和1 mmol/L浓度下暴露于ATP或ADO 48小时时，细胞周期阶段分布和增殖指数（PI）值发生变化。ATP和ADO通过使细胞周期阶段分布改变，经由G0/G1期（ATP或ADO，1 mmol/L）或S期（ATP，0.1 mmol/L）阻滞来抑制细胞增殖。在光学显微镜下，暴露于0.3 mmol/L ATP或ADO的肿瘤细胞呈现出凋亡的形态变化。在琼脂糖凝胶电泳中，用0.1 - 1 mmol/L ATP或ADO处理的TE - 13细胞获得了DNA片段化的梯状图谱。ATP和ADO在0.03至1 mmol/L浓度范围内以剂量依赖方式诱导TE - 13细胞凋亡。暴露于ATP或ADO 48小时的TE - 13细胞的最大凋亡率分别为16.63%或16.9%。
结论
ATP和ADO抑制TE - 13细胞系的细胞增殖，阻滞细胞周期并诱导其凋亡。
Zotero 插件