肝组织中特定单链DNA浓度作为丙型肝炎病毒感染患者细胞凋亡指标
Specific ssDNA concentration in liver tissue as an index of apoptosis in hepatitis C virus-infected patients.
作者信息
Lapinski Tadeusz-Wojciech, Panasiuk Anatol, Jaroszewicz Jerzy, Kowalczuk Oksana, Flisiak Robert, Rogalska Magdalena
机构信息
Department of Infectious Diseases, Medical University of Bialystok, Bialystok 15-540, Zurawia str, 14, Poland.
出版信息
World J Gastroenterol. 2005 Oct 21;11(39):6130-3. doi: 10.3748/wjg.v11.i39.6130.
AIM
To evaluate the activity of apoptosis in liver tissue and explore its possible association with hepatic necroinflammation and fibrosis as well as serum hepatitis C virus (HCV) load.
METHODS
The studied population included 50 chronic hepatitis C patients (20 women and 30 men, aged 18-66 years). HCV-RNA quantification was performed by two-step real-time quantitative RT-PCR method using the TaqMan technology (reagents of Applera Corporation firm, USA). The morphology of liver tissue was assessed descriptively and scored (necroinflammatory activity and fibrosis). The early apoptosis activity in liver tissue was examined by ssDNA apoptosis ELISA kit, (Chemicon, Germany).
RESULTS
The correlation between apoptosis and fibrosis in liver tissue was observed. High intensification of apoptosis was proportional to the increase of fibrosis (ssDNA: 16.65 x 10(-5) microug/g; 12.71 x 10(-5) microg/g), however, this difference was not statistically significant (P>0.05). Activity of apoptosis in the liver tissue, expressed by ssDNA concentration did not depend on hepatic necroinflammatory changes, HCV-RNA viral load, ALT, and AST activity as well as prothrombin time and INR index.
CONCLUSION
Fibrosis in the tissue is closely associated with early apoptosis in HCV-infected patients.
目的
评估肝组织中的细胞凋亡活性，并探讨其与肝坏死性炎症、纤维化以及血清丙型肝炎病毒（HCV）载量之间的可能关联。
方法
研究人群包括50例慢性丙型肝炎患者（20名女性和30名男性，年龄18 - 66岁）。使用TaqMan技术（美国Applera Corporation公司试剂）通过两步实时定量RT - PCR方法进行HCV - RNA定量。对肝组织形态进行描述性评估并评分（坏死性炎症活性和纤维化）。使用ssDNA细胞凋亡ELISA试剂盒（德国Chemicon公司）检测肝组织中的早期细胞凋亡活性。
结果
观察到肝组织中细胞凋亡与纤维化之间的相关性。细胞凋亡的高强度增加与纤维化的增加成比例（单链DNA：16.65×10⁻⁵微克/克；12.71×10⁻⁵微克/克），然而，这种差异无统计学意义（P>0.05）。以单链DNA浓度表示的肝组织细胞凋亡活性不依赖于肝坏死性炎症变化、HCV - RNA病毒载量、ALT和AST活性以及凝血酶原时间和INR指数。
结论
组织中的纤维化与HCV感染患者的早期细胞凋亡密切相关。
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