Liu Guangjie, Yang Liumeng, Wang Jianhua, Zhang Gaohong, Chen Xiangmei, Zheng Yongtang
Laboratory of Molecular Immunopharmacology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.
Cell Mol Immunol. 2005 Aug;2(4):289-93.
Many B cell epitopes within p24 of human immunodeficiency virus type 1 (HIV-1) were identified, while most of them were determined by using murine monoclonal antibodies reacting with overlapping peptides of p24. Therefore these epitopes may not represent the actual epitopes recognized by the HIV-1 infected individuals. In the present study, immune responses of 67 HIV-1 positive sera from Yunnan Province, China to five peptides on p24 of HIV-1 and one of HIV-2 were analyzed. All of 67 sera did not recognize peptide GA-12 on HIV-1 and peptide AG-23 on HIV-2, which indicated that GA-12 was not human B cell epitope and AG-23 did not cross-react with HIV-1 positive serum. Except 13 sera (19.4%), all remaining sera did not recognize peptides NI-15, DR-16, DC-22 and PS-18, which indicated that these four peptides represented B cell linear epitopes of HIV-1 p24 in some HIV-1 infected individuals but not the immuno-dominant epitopes in most individuals.
已鉴定出人类免疫缺陷病毒1型（HIV-1）p24内的许多B细胞表位，然而其中大多数是通过使用与p24重叠肽段反应的鼠单克隆抗体来确定的。因此，这些表位可能并不代表HIV-1感染个体所识别的实际表位。在本研究中，分析了来自中国云南省的67份HIV-1阳性血清对HIV-1 p24上的五种肽段以及一种HIV-2肽段的免疫反应。67份血清均未识别HIV-1上的肽段GA-12以及HIV-2上的肽段AG-23，这表明GA-12不是人类B细胞表位，且AG-23与HIV-1阳性血清无交叉反应。除13份血清（19.4%）外，其余所有血清均未识别肽段NI-15、DR-16、DC-22和PS-18，这表明这四种肽段在一些HIV-1感染个体中代表HIV-1 p24的B细胞线性表位，但并非大多数个体中的免疫显性表位。
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