Losytskyy M Yu, Volkova K D, Kovalska V B, Makovenko I E, Slominskii Yu L, Tolmachev O I, Yarmoluk S M
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, 03143 Kyiv, Ukraine.
J Fluoresc. 2005 Nov;15(6):849-57. doi: 10.1007/s10895-005-0002-7.
A series of pentamethine cyanine dyes with cyclohexene or cyclopentene group in polymethyne chain, assumed as DNA groove-binders, were studied as fluorescent probes for nucleic acids as well as for native and denatured proteins. It was revealed that the presence of methyl or dimethyl substituent in 5 position of the cyclohexene group hinders the formation of dye-DNA fluorescent complex, while the methyl substituent in 2 position leads to the increasing of the dye-DNA complex fluorescence intensity. The dyes SL-251, SL-1041, and SL-1046 containing methyl group in the 2 position of the cyclic group, are reported as bright DNA-sensitive dyes. The study of the dyes DNA-binding specificity demonstrated significant AT-preference that points to the groove-binding interaction mode. At the same time, the dyes SL-251, SL-377, and SL-957 with the 2-methyl substituted cyclohexene group were shown to be sensitive fluorescent dyes both for nonspecific (in SDS presence) proteins detection and for native BSA.
研究了一系列在聚甲炔链中带有环己烯或环戊烯基团的五甲川菁染料，它们被认为是DNA沟结合剂，作为核酸以及天然和变性蛋白质的荧光探针进行了研究。结果表明，环己烯基团5位上存在甲基或二甲基取代基会阻碍染料-DNA荧光复合物的形成，而2位上的甲基取代基会导致染料-DNA复合物荧光强度增加。含有环基团2位甲基的染料SL-251、SL-1041和SL-1046被报道为明亮的DNA敏感染料。对染料DNA结合特异性的研究表明，其对AT有明显偏好，这表明其沟结合相互作用模式。同时，带有2-甲基取代环己烯基团的染料SL-251、SL-377和SL-957被证明是对非特异性（在SDS存在下）蛋白质检测和天然牛血清白蛋白都敏感的荧光染料。
