Olenych Scott G, Moussallem Maroun D, Salloum David S, Schlenoff Joseph B, Keller Thomas C S
Department of Biological Science, The Florida State University, Tallahassee, Florida 32306, USA.
Biomacromolecules. 2005 Nov-Dec;6(6):3252-8. doi: 10.1021/bm050298r.
Culture of A7r5 smooth muscle cells on a polyelectrolyte multilayer film (PEMU) can influence various cell properties, including adhesion, motility, and cytoskeletal organization, that are modulated by the extracellular matrix (ECM) in vivo. ECM contribution to cell behavior on PEMUs was investigated by determining the amount of fibronectin (FN) bound to charged and hydrophobic PEMUs by optical waveguide lightmode spectroscopy and immunofluorescence microscopy. FN bound best to poly(allylamine hydrochloride) (PAH)-terminated and Nafion-terminated PEMUs. FN bound poorly to PEMUs terminated with a copolymer of poly(acrylic acid) (PAA) and 3-[2-(acrylamido)-ethyl dimethylammonio] propane sulfonate (PAA-co-AEDAPS). Cells adhered and spread well on the Nafion-terminated PEMU surfaces. In contrast, cells spread less and migrated more on both FN-coated and uncoated PAH-terminated PEMU surfaces. Both cells and FN interacted much better with Nafion than with PAA-co-PAEDAPS in a micropatterned PEMU. These results indicate that A7r5 cell adhesion, spreading, and motility on PEMUs can be independent of FN binding to the surfaces.
在聚电解质多层膜（PEMU）上培养A7r5平滑肌细胞会影响多种细胞特性，包括黏附、运动以及细胞骨架组织，这些特性在体内会受到细胞外基质（ECM）的调节。通过光波导光模式光谱法和免疫荧光显微镜测定结合到带电和疏水PEMU上的纤连蛋白（FN）的量，研究了ECM对PEMU上细胞行为的影响。FN与聚（烯丙胺盐酸盐）（PAH）封端和Nafion封端的PEMU结合最佳。FN与用聚（丙烯酸）（PAA）和3-[2-（丙烯酰胺基）-乙基二甲基铵]丙烷磺酸盐（PAA-co-AEDAPS）的共聚物封端的PEMU结合较差。细胞在Nafion封端的PEMU表面上黏附并铺展良好。相比之下，细胞在FN包被和未包被的PAH封端的PEMU表面上铺展较少且迁移较多。在微图案化的PEMU中，细胞和FN与Nafion的相互作用都比与PAA-co-PAEDAPS的相互作用好得多。这些结果表明，A7r5细胞在PEMU上的黏附、铺展和运动可能与FN与表面的结合无关。
