Shende Niraj, Gupta Sonika, Kumar Satish, Harinath B C
Department of Biochemistry, Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, Maharashtra.
Indian J Pathol Microbiol. 2004 Jul;47(3):438-40.
The ES-31 (31 kDa protein) antigen was isolated from culture filtrate of Mycobacterium tuberculosis H37Ra and was shown to have potential in immunodiagnosis of pulmonary tuberculosis. Serum samples from 38 confirmed sputum positive pulmonary tuberculosis patients were grouped into AFB+, AFB++, AFB+++ based on sputum bacillary load. Analysis of tubercular antibody, circulating free and immune complexed antigen (CIC-Ag) was done in serum samples by Indirect and Sandwich ELISA using ES-31 antigen and affinity purified anti ES-31 antibody respectively. The analysis of Geometric mean titre (GMT) of all the three groups showed that GMT of tubercular antibody was considerably decreased compared to elevated levels of CIC-Ag (Antibody: 1360 to 816 and CIC-Ag: 534 to 1744) from low bacillary sample to high bacillary samples, whereas there is no significant change in the titre of circulating free antigen. Low levels of detectable antibody is observed possibly due to removal of antibody from circulation by immune complex formation as confirmed by its elevated levels in sputum AFB+++ positive patients.
ES-31（31 kDa蛋白）抗原是从结核分枝杆菌H37Ra的培养滤液中分离出来的，已证明其在肺结核免疫诊断中具有潜力。将38例确诊为痰涂片阳性的肺结核患者的血清样本根据痰菌载量分为AFB+、AFB++、AFB+++组。分别使用ES-31抗原和亲和纯化的抗ES-31抗体，通过间接ELISA和夹心ELISA对血清样本中的结核抗体、循环游离抗原和免疫复合物抗原（CIC-Ag）进行分析。对所有三组的几何平均滴度（GMT）分析表明，从低菌量样本到高菌量样本，结核抗体的GMT显著降低，而CIC-Ag水平升高（抗体：1360至816，CIC-Ag：534至1744），而循环游离抗原的滴度没有显著变化。观察到可检测抗体水平较低，可能是由于免疫复合物形成导致抗体从循环中清除，痰AFB+++阳性患者中抗体水平升高证实了这一点。
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