[溶血磷脂酸受体在人卵巢癌细胞系3AO、SKOV3、OVCAR3中的表达及其意义]
[Expression of lysophosphatidic acid receptor in human ovarian cancer cell lines 3AO, SKOV3, OVCAR3 and its significance].
作者信息
Wang Ping, Wu Xiao-hua, Chen Wen-xue, Shan Bao-en, Guo Qing
机构信息
Department of Obstetrics and Gynecology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2005 Nov;25(11):1422-4, 1431.
OBJECTIVE
To investigate the expressions of lysophosphatidic acid (LPA) receptors at both mRNA and protein levels and their biological significance in different human ovarian cancer cell lines.
METHODS
Reverse transcription-polymerase chain reaction (RT-PCR), streptovidin peroxidase-conjugated (SP) immunohistochemical assay and Western blotting were employed to measure the expression levels of LPA(1), LPA(2) and LPA(3) mRNA and LPA(2) and LPA(3) protein in cultured human ovarian cancer cell lines 3AO.SKOV3, and OVCAR3.
RESULTS
All the ovarian cancer cell lines expressed LPA(1), LPA(2) and LPA(3) mRNAs and LPA(2) and LPA(3) proteins.
CONCLUSION
LPA(1), LPA(2) and LPA(3) expressions occur extensively in human epithelial ovarian cancer cell lines, and LPA(2) and LPA(3) may be involved in the development and progression of human ovarian cancer.
目的
研究溶血磷脂酸（LPA）受体在不同人卵巢癌细胞系中的mRNA和蛋白水平表达及其生物学意义。
方法
采用逆转录-聚合酶链反应（RT-PCR）、链霉亲和素过氧化物酶结合物（SP）免疫组织化学检测法和蛋白质印迹法，检测培养的人卵巢癌细胞系3AO、SKOV3和OVCAR3中LPA(1)、LPA(2)和LPA(3) mRNA以及LPA(2)和LPA(3)蛋白的表达水平。
结果
所有卵巢癌细胞系均表达LPA(1)、LPA(2)和LPA(3) mRNA以及LPA(2)和LPA(3)蛋白。
结论
LPA(1)、LPA(2)和LPA(3)在人上皮性卵巢癌细胞系中广泛表达，LPA(2)和LPA(3)可能参与人卵巢癌的发生发展。
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