氯化亚锡的相互作用会导致DNA、三磷酸核苷酸和分离碱基发生改变。

Interaction of stannous chloride leads to alteration in DNA, triphosphate nucleotides and isolated bases.

作者信息

de Mattos José C P, Lage Claudia, Dantas Flávio J S, Moraes Milton O, Nunes Ana P M, Bezerra Roberto J A C, Faria Mauro V Castro, Leitão Alvaro C, Caldeira-de-Araujo Adriano

机构信息

Departamento de Biofísica e Biometria, IBRAG, UERJ, Avenue 28 de Setembro, 87, Rio de Janeiro, RJ, 20551-030, Brazil.

出版信息

Mol Cell Biochem. 2005 Dec;280(1-2):173-9. doi: 10.1007/s11010-005-8908-6.

DOI:10.1007/s11010-005-8908-6

PMID:16311920

Abstract

Stannous chloride (SnCl2) is a reducing chemical agent used in several man-made products. SnCl2 can generate reactive oxygen species (ROS); therefore, studies have been carried out in order to better understand its damaging action in biological systems. In this work, calf thymus DNA, triphosphate nucleotides and isolated bases were incubated with SnCl2 and the results were analyzed through UV spectrophotometry. The presence of stannous ions altered the absorption spectra of all three isolates. The amount of stannous ions associated to DNA was measured by atomic absorption spectrophotometry. Data showed that more than 40% of the initial SnCl2 concentration was present in the samples. Our results are in accordance with the damaging potential of this salt and present evidence that stannous ions can complex with DNA, inducing ROS in its vicinity, which may be responsible for the observed lesions.

摘要

氯化亚锡（SnCl₂）是一种用于多种人造产品的还原性化学试剂。SnCl₂ 可产生活性氧（ROS）；因此，人们开展了多项研究以更好地了解其在生物系统中的破坏作用。在这项工作中，将小牛胸腺 DNA、三磷酸核苷酸和分离出的碱基与 SnCl₂ 一起孵育，并通过紫外分光光度法分析结果。亚锡离子的存在改变了所有三种分离物的吸收光谱。通过原子吸收分光光度法测量与 DNA 结合的亚锡离子量。数据显示，样品中存在超过 40% 的初始 SnCl₂ 浓度。我们的结果与这种盐的破坏潜力相符，并表明亚锡离子可与 DNA 结合，在其附近产生活性氧，这可能是观察到的损伤的原因。