前房相关免疫偏离发展过程中GATA-3的表达

GATA-3 expression in the development of anterior chamber associated immune deviation.

作者信息

Fu Tao, Yang Pei-zeng, Huang Xiang-kun, Huang Qiang, Zhou Hong-yan, Li Bing, Zhong Hua-hong, Chen Xuan

机构信息

Uveitis Study Center, Sun Yat-sen University, Guangzhou 510060, China.

出版信息

Chin Med J (Engl). 2005 Dec 5;118(23):2000-4.

PMID:16336837

Abstract

BACKGROUND

Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.

METHODS

ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS

A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.

CONCLUSION

The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.

摘要

背景

前房相关免疫偏离（ACAID）的特征为Th2细胞反应。已证明GATA-3对Th2细胞的激活是必需的。本研究旨在检测GATA-3在ACAID发生过程中的表达情况。

方法

通过向Wistar大鼠前房注射50微克光感受器间维生素A结合蛋白（IRBP）诱导ACAID。在接种IRBP后的第3、7、14、21、28天评估迟发型超敏反应（DTH）。采用免疫组织化学染色检测GATA-3表达。通过逆转录聚合酶链反应（RT-PCR）检测IRBP前房注射后不同时间点GATA-3 mRNA的表达。