• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

谷胱甘肽对顺式-[PtCl2(13CH3NH2)2]的转位作用可在生理pH值下通过[1H,13C] HSQC NMR进行监测。

The trans labilization of cis-[PtCl2(13CH3NH2)2] by glutathione can be monitored at physiological pH by [1H,13C] HSQC NMR.

作者信息

Gibson Dan, Kasherman Yonit, Kowarski Dina, Freikman Inna

机构信息

Department of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University of Jerusalem, P.O. Box 12065, 91120 Jerusalem, Israel.

出版信息

J Biol Inorg Chem. 2006 Mar;11(2):179-88. doi: 10.1007/s00775-005-0064-7. Epub 2005 Dec 10.

DOI:10.1007/s00775-005-0064-7
PMID:16341898
Abstract

In order to monitor the trans labilization of cisplatin at physiological pH we have prepared the complex cis-[PtCl(2)((13)CH(3)NH(2))(2)] and studied its interactions with excess glutathione in aqueous solution at neutral pH by two-dimensional [1H,13C] heteronuclear single-quantum correlation (HSQC) NMR spectroscopy. [1H,13C] HSQC spectroscopy is a good method for following the release of (13)CH(3)NH(2) but is not so good for characterizing the Pt species in solution. In the reaction of cisplatin with glutathione, Pt-S bonds are formed and Pt-NH(3) bonds are broken. The best technique for following the formation of Pt-S bonds of cisplatin is by UV spectroscopy. [1H,13C] HSQC spectroscopy is the best method for following the breaking of the Pt-N bonds. [1H,15N] HSQC spectroscopy is the best method for characterizing the different species in solution. However, the intensity of the peaks in the (15)NH(3)-Pt-S region, in [1H,15N] HSQC, reflects a balance between the formation of Pt-S bonds, which increases the signal intensity, and the trans labilization, which decreases the signal intensity. [1H,15N] HSQC spectroscopy and [1H,13C] HSQC spectroscopy are complementary techniques that should be used in conjunction in order to obtain the most accurate information on the interaction of platinum complexes with sulfur-containing ligands.

摘要

为了监测顺铂在生理pH值下的转位作用，我们制备了配合物顺式-[PtCl₂((¹³)CH₃NH₂)₂]，并通过二维[¹H,¹³C]异核单量子相关（HSQC）核磁共振光谱研究了其在中性pH值的水溶液中与过量谷胱甘肽的相互作用。[¹H,¹³C] HSQC光谱是追踪(¹³)CH₃NH₂释放的好方法，但对于表征溶液中的铂物种并不理想。在顺铂与谷胱甘肽的反应中，形成了Pt-S键，Pt-NH₃键断裂。追踪顺铂Pt-S键形成的最佳技术是紫外光谱法。[¹H,¹³C] HSQC光谱是追踪Pt-N键断裂的最佳方法。[¹H,¹⁵N] HSQC光谱是表征溶液中不同物种的最佳方法。然而，在[¹H,¹⁵N] HSQC中，(¹⁵)NH₃-Pt-S区域峰的强度反映了Pt-S键形成（增加信号强度）和转位作用（降低信号强度）之间的平衡。[¹H,¹⁵N] HSQC光谱和[¹H,¹³C] HSQC光谱是互补技术，应结合使用以获得关于铂配合物与含硫配体相互作用的最准确信息。

相似文献

1
The trans labilization of cis-[PtCl2(13CH3NH2)2] by glutathione can be monitored at physiological pH by [1H,13C] HSQC NMR.谷胱甘肽对顺式-[PtCl2(13CH3NH2)2]的转位作用可在生理pH值下通过[1H,13C] HSQC NMR进行监测。
J Biol Inorg Chem. 2006 Mar;11(2):179-88. doi: 10.1007/s00775-005-0064-7. Epub 2005 Dec 10.
2
Reaction of polynuclear platinum antitumor compounds with reduced glutathione studied by multinuclear (1H, 1H-15N gradient heteronuclear single-quantum coherence, and 195Pt) NMR spectroscopy.通过多核（¹H、¹H-¹⁵N梯度异核单量子相干和¹⁹⁵Pt）核磁共振波谱研究多核铂抗肿瘤化合物与还原型谷胱甘肽的反应。
Inorg Chem. 2003 Sep 8;42(18):5498-506. doi: 10.1021/ic030045b.
3
Reaction of cis- and trans-[PtCl2(NH3)2] with reduced glutathione inside human red blood cells, studied by 1H and 15N-[1H] DEPT NMR.通过¹H和¹⁵N-[¹H] DEPT核磁共振研究顺式和反式-[PtCl₂(NH₃)₂]与人红细胞内还原型谷胱甘肽的反应。
J Inorg Biochem. 1990 Apr;38(4):327-45. doi: 10.1016/0162-0134(90)80006-j.
4
Equilibrium and kinetic studies of the aquation of the dinuclear platinum complex [[trans-PtCl(NH3)2]2(mu-NH2(CH2)6NH2)]2+: pKa determinations of aqua ligands via [1H,15N] NMR spectroscopy.双核铂配合物[[反式-PtCl(NH₃)₂]₂(μ-NH₂(CH₂)₆NH₂)]²⁺水合反应的平衡与动力学研究：通过[¹H,¹⁵N]核磁共振光谱法测定水合配体的pKa值
Inorg Chem. 2000 Apr 17;39(8):1710-5. doi: 10.1021/ic991104h.
5
The trans influence in the modulation of platinum anticancer agent biology: the effect of nitrite leaving group on aquation, reactions with S-nucleophiles and DNA binding of dinuclear and trinuclear compounds.反式影响铂类抗癌药物的生物学作用：亚硝酸盐离去基团对水合作用、与 S-亲核试剂的反应以及双核和三核化合物与 DNA 结合的影响。
Chemistry. 2010 Aug 9;16(30):9175-85. doi: 10.1002/chem.200903578.
6
Trans labilization of am(m)ine ligands from platinum(II) complexes by cancer cell extracts.癌细胞提取物对铂（II）配合物中氨（胺）配体的转位作用。
J Biol Inorg Chem. 2009 Mar;14(3):387-99. doi: 10.1007/s00775-008-0456-6. Epub 2008 Dec 4.
7
Kinetic and equilibria studies of the aquation of the trinuclear platinum phase II anticancer agent [(trans-PtCl(NH(3))(2))(2)(mu-trans-Pt(NH(3))(2)(NH(2)(CH(2))(6)NH(2))(2))](4+) (BBR3464).三核铂II期抗癌药物[(反式-PtCl(NH₃)₂)₂(μ-反式-Pt(NH₃)₂(NH₂(CH₂)₆NH₂)₂)]⁴⁺（BBR3464）水合反应的动力学和平衡研究
Inorg Chem. 2002 Mar 11;41(5):1101-9. doi: 10.1021/ic010851n.
8
Effects of geometric isomerism in dinuclear antitumor platinum complexes on their interactions with N-acetyl-L-methionine.双核抗肿瘤铂配合物中几何异构对其与N-乙酰-L-蛋氨酸相互作用的影响。
J Biol Inorg Chem. 2005 Aug;10(5):433-42. doi: 10.1007/s00775-005-0009-1. Epub 2005 Sep 23.
9
Pt(II)-mediated nitrile-tetramethylguanidine coupling as a key step for a novel synthesis of 1,6-dihydro-1,3,5-dihydrotriazines.铂（II）介导的腈与四甲基胍偶联反应作为新型合成1,6-二氢-1,3,5-二氢三嗪的关键步骤。
Inorg Chem. 2007 Mar 5;46(5):1684-93. doi: 10.1021/ic061884b. Epub 2007 Feb 1.
10
Reactions of cisplatin hydrolytes with methionine, cysteine, and plasma ultrafiltrate studied by a combination of HPLC and NMR techniques.通过高效液相色谱法和核磁共振技术相结合的方法研究顺铂水解产物与蛋氨酸、半胱氨酸及血浆超滤液的反应。
J Inorg Biochem. 1999 Oct;77(1-2):13-21. doi: 10.1016/s0162-0134(99)00146-4.

引用本文的文献

1
Exploring the Structure-Activity Relationships of Albumin-Targeted Picoplatin-Based Platinum(IV) Prodrugs.探索白蛋白靶向的基于吡啶铂的铂（IV）前药的构效关系。
Inorg Chem. 2025 Feb 10;64(5):2554-2566. doi: 10.1021/acs.inorgchem.4c05269. Epub 2025 Jan 29.
2
Cisplatin handover between copper transporters: the effect of reducing agents.顺铂在铜转运蛋白间的传递：还原剂的影响。
J Biol Inorg Chem. 2014 Jun;19(4-5):705-14. doi: 10.1007/s00775-014-1138-1. Epub 2014 May 1.
3
Determination of the kinetic profile of a dinuclear platinum anticancer complex in the presence of sulfate: introducing a new tool for the expedited analysis of 2D [(1)H,( 15)N] HSQC NMR spectra.

本文引用的文献

1
Cellular processing of platinum anticancer drugs.铂类抗癌药物的细胞处理过程。
Nat Rev Drug Discov. 2005 Apr;4(4):307-20. doi: 10.1038/nrd1691.
2
Biochemical modulation of Cisplatin mechanisms of action: enhancement of antitumor activity and circumvention of drug resistance.顺铂作用机制的生化调节：增强抗肿瘤活性并规避耐药性。
Chem Rev. 2003 Mar;103(3):645-62. doi: 10.1021/cr020010d.
3
Kinetic study of the reaction of cisplatin with thiols.顺铂与硫醇反应的动力学研究。
测定双核铂抗癌配合物在硫酸存在下的动力学特征：引入一种新的工具，用于加速二维 [(1)H,( 15)N] HSQC NMR 谱的分析。
Inorg Chem. 2010 Dec 6;49(23):10815-9. doi: 10.1021/ic100576k. Epub 2010 Nov 10.
4
The trans influence in the modulation of platinum anticancer agent biology: the effect of nitrite leaving group on aquation, reactions with S-nucleophiles and DNA binding of dinuclear and trinuclear compounds.反式影响铂类抗癌药物的生物学作用：亚硝酸盐离去基团对水合作用、与 S-亲核试剂的反应以及双核和三核化合物与 DNA 结合的影响。
Chemistry. 2010 Aug 9;16(30):9175-85. doi: 10.1002/chem.200903578.
5
Cisplatin interaction with phosphatidylserine bilayer studied by solid-state NMR spectroscopy.顺铂与磷脂酰丝氨酸双层的固态 NMR 光谱研究。
J Biol Inorg Chem. 2010 Feb;15(2):213-23. doi: 10.1007/s00775-009-0586-5. Epub 2009 Sep 19.
6
Trans labilization of am(m)ine ligands from platinum(II) complexes by cancer cell extracts.癌细胞提取物对铂（II）配合物中氨（胺）配体的转位作用。
J Biol Inorg Chem. 2009 Mar;14(3):387-99. doi: 10.1007/s00775-008-0456-6. Epub 2008 Dec 4.
Drug Metab Dispos. 2002 Dec;30(12):1378-84. doi: 10.1124/dmd.30.12.1378.
4
Novel soluble cationic trans-diaminedichloroplatinum(II) complexes that are active against cisplatin resistant ovarian cancer cell lines.
J Med Chem. 2002 Nov 21;45(24):5189-95. doi: 10.1021/jm0201969.
5
Molecular aspects of resistance to antitumor platinum drugs.抗肿瘤铂类药物耐药性的分子机制
Drug Resist Updat. 2002 Jul-Aug;5(3-4):147-61. doi: 10.1016/s1368-7646(02)00047-x.
6
Kinetics of cisplatin binding to cellular DNA and modulations by thiol-blocking agents and thiol drugs.顺铂与细胞DNA结合的动力学以及硫醇阻断剂和硫醇类药物的调节作用。
Drug Metab Dispos. 2002 Feb;30(2):183-90. doi: 10.1124/dmd.30.2.183.
7
Structure, Recognition, and Processing of Cisplatin-DNA Adducts.顺铂-DNA加合物的结构、识别与处理
Chem Rev. 1999 Sep 8;99(9):2467-98. doi: 10.1021/cr980421n.
8
Current status of platinum-based antitumor drugs.铂类抗肿瘤药物的现状
Chem Rev. 1999 Sep 8;99(9):2451-66. doi: 10.1021/cr980420v.
9
Ring-Opened Adducts of the Anticancer Drug Carboplatin with Sulfur Amino Acids.抗癌药物卡铂与含硫氨基酸的开环加合物
Inorg Chem. 1996 Feb 14;35(4):1065-1072. doi: 10.1021/ic950973d.
10
Cisplatin: from DNA damage to cancer chemotherapy.顺铂：从DNA损伤到癌症化疗
Prog Nucleic Acid Res Mol Biol. 2001;67:93-130. doi: 10.1016/s0079-6603(01)67026-0.