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通过与黄孢原毛平革菌和秸秆共同培养对铅污染土壤进行生物修复。

Bioremediation of Pb-contaminated soil by incubating with Phanerochaete chrysosporium and straw.

作者信息

Huang Dan-Lian, Zeng Guang-Ming, Jiang Xiao-Yun, Feng Chong-Ling, Yu Hong-Yan, Huang Guo-He, Liu Hong-Liang

机构信息

College of Environmental Science and Engineering, Hunan University, Changsha 410082, Hunan, China.

出版信息

J Hazard Mater. 2006 Jun 30;134(1-3):268-76. doi: 10.1016/j.jhazmat.2005.11.021. Epub 2005 Dec 15.

Abstract

The bioremediation of the simulated lead (Pb)-contaminated soils by incubating with Phanerochaete chrysosporium and straw was studied at laboratory-scale. The soil pH, Pb concentration, soil microbial biomass, microbial metabolic quotient, microbial quotient and microbial biomass C-to-N ratios were monitored. The above indicators were to study the stress of Pb on soil and the microbial effects during the bioremediation process. It was found that the soils treated with P. chrysosporium and straw showed a much lower concentration of soluble-exchangeable Pb, lower metabolic quotient and biomass C-to-N ratios (0mgkg(-1) dry weight soil, 1.9mg CO(2)-Cmg(-1) biomass carbon and 4.9 on day 60, respectively) and higher microbial biomass and microbial quotient (2258mgkg(-1) dry weight soil and 7.86% on day 60, respectively) compared with the controls. In addition, the kinetic parameters in the model based on logistic equation were calculated by the BIOLOG data. By analyzing those kinetic parameters some information on the metabolic capacity of the microbial community could be obtained. All the results indicated that the bioavailability of Pb in contaminated soil was reduced so that the potential stress of Pb was alleviated, and also showed that the soil microbial effects and the metabolic capacity of microbial community were improved.

摘要

在实验室规模下,研究了通过与黄孢原毛平革菌和秸秆一起培养对模拟铅(Pb)污染土壤进行生物修复的情况。监测了土壤pH值、铅浓度、土壤微生物生物量、微生物代谢商、微生物商和微生物生物量碳氮比。上述指标用于研究铅对土壤的胁迫以及生物修复过程中的微生物效应。结果发现,与对照相比,用黄孢原毛平革菌和秸秆处理的土壤中可溶性交换态铅浓度更低、代谢商和生物量碳氮比更低(分别在第60天为0mgkg(-1)干重土壤、1.9mg CO(2)-Cmg(-1)生物量碳和4.9),而微生物生物量和微生物商更高(分别在第60天为2258mgkg(-1)干重土壤和7.86%)。此外,利用BIOLOG数据计算了基于逻辑方程模型中的动力学参数。通过分析这些动力学参数,可以获得有关微生物群落代谢能力的一些信息。所有结果表明,污染土壤中铅的生物有效性降低,从而减轻了铅的潜在胁迫,同时也表明土壤微生物效应和微生物群落的代谢能力得到了改善。

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