Department of Agricultural Engineering, University of Missouri-Columbia, and Science and Education Administration, U.S. Department of Agriculture, Columbia, Missouri 65211.
Appl Environ Microbiol. 1982 Jan;43(1):247-9. doi: 10.1128/aem.43.1.247-249.1982.
Most bacterial strains isolated from a swine manure digester grew sufficiently to permit transfer of cultures, but not characterization. Substrates, crude extracts, growth factors, and electron acceptors were evaluated for growth promotion. The growth of all but one group of the isolates was substantially increased with a medium containing glucose, cellobiose, soluble starch, pyruvate, peptone, yeast extract, minerals, volatile acids, vitamins, hemin plus vitamins K(1) and K(3), sodium bicarbonate, cysteine, and digester fluid. The strains require both known and unknown factors (in crude extracts) for maximal growth.
从猪粪消化器中分离出的大多数细菌菌株都能够充分生长,从而允许进行培养物的转移,但无法进行特征描述。评估了基质、粗提取物、生长因子和电子受体对生长的促进作用。除一组分离物外,所有分离物的生长都在含有葡萄糖、纤维二糖、可溶性淀粉、丙酮酸盐、蛋白胨、酵母提取物、矿物质、挥发性酸、维生素、血红素加维生素 K(1)和 K(3)、碳酸氢钠、半胱氨酸和消化器液体的培养基中得到了显著提高。这些菌株需要已知和未知的因素(在粗提取物中)才能实现最大生长。