根瘤菌培养上清液中吲哚-3-乙酸的质谱定量分析：与根毛卷曲和根瘤起始的关系。
Mass spectrometric quantification of indole-3-acetic Acid in Rhizobium culture supernatants: relation to root hair curling and nodule initiation.
机构信息
Department of Developmental Biology, Department of Genetics, and Department of Environmental Biology, Research School of Biological Sciences, Australian National University, Canberra City, A.C.T. 2601, Australia.
出版信息
Appl Environ Microbiol. 1982 Aug;44(2):275-80. doi: 10.1128/aem.44.2.275-280.1982.
Abstract
Indole-3-acetic acid (IAA) has been unequivocally identified in culture supernatants of Rhizobium strains by gas chromatography-mass spectrometry. A method for accurately quantitating IAA in bacterial culture supernatants, employing deuterium-labeled IAA as an internal standard, has been developed. Similar IAA concentrations were found in culture supernatants of chosen Rhizobium mutants (defective in nodule formation) and their corresponding parent strains. Since some of the mutants are known to adhere to root hairs, it can be concluded that root hair curling is not simply a consequence of IAA production by rhizobia.
摘要
吲哚-3-乙酸（IAA）已通过气相色谱-质谱法在根瘤菌菌株的培养上清液中得到明确鉴定。已经开发出一种用于准确定量细菌培养上清液中 IAA 的方法，该方法采用氘标记的 IAA 作为内标。在选定的根瘤菌突变体（在形成根瘤方面有缺陷）及其相应的亲本菌株的培养上清液中发现了类似浓度的 IAA。由于已知某些突变体能够附着在根毛上，因此可以得出结论，根毛卷曲不仅仅是根瘤菌产生 IAA 的结果。
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