Dairy Research Laboratory, Division of Food Research, Commonwealth Scientific and Industrial Research Organization, Highett, Victoria, Australia 3190.
Appl Environ Microbiol. 1983 Jun;45(6):1946-8. doi: 10.1128/aem.45.6.1946-1948.1983.
When M17 broth was used as growth medium before preparation of cell walls, adsorption of phage eb7 on Streptococcus cremoris EB7 at pH 4.0 was stimulated. When preparation included treatment with trypsin, absorption of phage was 65%. Without trypsin treatment, absorption was 81%. Only 10 to 20% of the adsorption was irreversible. Treatment with pepsin or commercial rennet but not pure chymosin prevented adsorption on non-trypsin-treated cell walls. d-Galactosamine treatment of phage eb7 had an inactivating effect which was enhanced by l-rhamnose.
当 M17 肉汤在细胞壁制备之前用作生长培养基时,噬菌体 eb7 在 pH 值为 4.0 时对嗜热链球菌 EB7 的吸附得到了刺激。当制备包括用胰蛋白酶处理时,噬菌体的吸收为 65%。没有胰蛋白酶处理时,吸收为 81%。只有 10%至 20%的吸附是不可逆的。用胃蛋白酶或商业凝乳酶处理,但不用纯凝乳酶处理,可以防止在未经胰蛋白酶处理的细胞壁上的吸附。d-半乳糖胺处理噬菌体 eb7 具有灭活作用,而 l-鼠李糖可以增强这种作用。