Chabardès D, Imbert M, Clique A, Montégut M, Morel F
Pflugers Arch. 1975;354(3):229-39. doi: 10.1007/BF00584646.
PTH sensitive adenylate cyclase activity was measured in 9 different segments of the nephron, isolated by microdissection from collagenase-treated rabbit kidney slices. The enzyme of the following segments was stimulated by PTH, 1 U/ml: PCT. (proximal convoluted tubule); PR (pars recta); CAL (cortical portion of the thick ascending limb); DCT (distal convoluted tubule); BCT (first, branched portion of the collecting tubule); the segments which did not respond to PTH were: TDL (thin descending limb): MAL (medullary portion of the thick ascending limb); CCT (cortical portion of the collecting tubule distally adjacent to BCT); MCT (collecting tubule from the outer medulla). PTH sensitive adenylate cyclase per mm tubule in PR was half that measured in PCT. Half maximal stimulation corresponded to 50-100mm U/ml PTH (1-2 times 10-8M) in both PCT and PR, and to about 350 mm U/ml in CAL. PTH (1 U/ml) stimulation factors ranged from 5 to 60 depending on the structures. It is concluded that in addition to PCT and PR, CAL and BCT might be target structures involved in the physiological actions of PTH on the kidney.
通过显微解剖从经胶原酶处理的兔肾切片中分离出肾单位的9个不同节段，测定甲状旁腺激素（PTH）敏感性腺苷酸环化酶活性。以下节段的酶受到1 U/ml PTH的刺激：近端曲管（PCT）；直部（PR）；厚升支皮质部（CAL）；远端曲管（DCT）；集合管第一分支部（BCT）；对PTH无反应的节段有：细降支（TDL）；厚升支髓质部（MAL）；紧邻BCT远端的集合管皮质部（CCT）；外髓集合管（MCT）。PR中每毫米肾小管的PTH敏感性腺苷酸环化酶活性是PCT中测得值的一半。在PCT和PR中，半数最大刺激对应50 - 100 mU/ml PTH（1 - 2×10⁻⁸M），在CAL中约为350 mU/ml。1 U/ml PTH的刺激因子根据结构不同在5到60之间。结论是，除了PCT和PR外，CAL和BCT可能是PTH对肾脏生理作用的靶结构。
