利用乙酸作为唯一主要碳源从土壤中选择性分离球形芽孢杆菌。
Selective Isolation of Bacillus sphaericus from Soil by Use of Acetate as the Only Major Source of Carbon.
机构信息
Department of Microbiology, The University, Western Bank, Sheffield S10 2TN, United Kingdom.
出版信息
Appl Environ Microbiol. 1985 Jun;49(6):1478-81. doi: 10.1128/aem.49.6.1478-1481.1985.
Abstract
A simple chemically defined medium containing sodium acetate (37 or 74 mM) as the only major source of carbon was inoculated with soil from various locations. The bacteria which grew most rapidly at 30 degrees C were almost entirely strains of Bacillus sphaericus and Arthrobacter species. Pasteurization of the soils made the medium selective for B. sphaericus. Several new strains were isolated, and the peptidoglycans of their cell walls were examined.
摘要
一种简单的化学成分确定的培养基，其中含有醋酸钠（37 或 74mM）作为唯一的主要碳源，接种于取自不同地点的土壤。在 30℃下生长最快的细菌几乎完全是球形芽孢杆菌和节杆菌属的菌株。土壤的巴氏消毒法使该培养基对球形芽孢杆菌具有选择性。分离出了几个新菌株，并对其细胞壁的肽聚糖进行了检查。
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