构建一种简单、廉价的多酶联免疫吸附试验微量滴定板清洗器。
Construction of a simple, inexpensive multiple enzyme-linked immunosorbent assay microdilution plate washer.
机构信息
Vineland Research Station, Agriculture Canada, Vineland Station, Ontario, Canada L0R 2E0.
出版信息
Appl Environ Microbiol. 1990 Jun;56(6):1763-7. doi: 10.1128/aem.56.6.1763-1767.1990.
In this paper, plans are given for the construction of an inexpensive enzyme-linked immunosorbent assay plate washer from readily available materials. The wash unit uses an intermittent wash cycle based on a wash manifold cycling over the microdilution plates for a predetermined time. Laboratory tests showed that the unit provided reliable, rapid washing of plates with tap water, with no detectable contamination between wells. Substrate absorbance values for test samples from machine-washed plates were equal to or greater than absorbance values for corresponding samples from plates washed manually by an accepted protocol, by using either enzyme-linked immunosorbent assay wash buffer or tap water.
本文提出了用易得材料制作廉价酶联免疫吸附测定板清洗器的方案。该清洗单元采用基于清洗歧管的间歇清洗循环，在预定时间内对微孔板进行循环冲洗。实验室测试表明，该单元使用自来水能够可靠、快速地清洗板，各孔之间没有可检测到的污染。使用酶联免疫吸附测定清洗缓冲液或自来水，从机洗板获得的测试样品的底物吸光度值与按照公认方案人工清洗板获得的相应样品的吸光度值相等或更高。
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