110 血清群大豆根瘤菌菌株的基因型多样性。
Genotypic Diversity among Strains of Bradyrhizobium japonicum Belonging to Serogroup 110.
机构信息
Soybean and Alfalfa Research Laboratory, Agricultural Research Service, HH-19, Building 011, BARC-West, U.S. Department of Agriculture, Beltsville, Maryland 20705; Department of Agronomy, University of Maryland, College Park, Maryland 20742 ; and Department of Botany, Zagazig University, Cairo, Egypt.
出版信息
Appl Environ Microbiol. 1993 Sep;59(9):3130-3. doi: 10.1128/aem.59.9.3130-3133.1993.
Thirty-three strains of Bradyrhizobium japonicum within serogroup 110 were examined for genotypic diversity by using DNA-DNA hybridization analyses. The analysis of the DNA from 15 hydrogen-uptake-negative strains with the bradyrhizobial uptake hydrogenase probe pHU52 showed variation in degree of homology and restriction fragment length polymorphism of EcoRI-restricted DNA. Clustering analysis of the 33 strains on the basis of DNA-DNA hybridization analysis with four restriction enzymes and with the bradyrhizobial nodulation locus, pRJUT10, as probe indicated the existence of four groups of strains, which were less than 70% similar. Restriction digestion of genomic DNA with BamHI and DNA-DNA hybridization with pRJUT10 permitted classification of each of the strains according to a specific fingerprint pattern.
通过 DNA-DNA 杂交分析，对血清群 110 内的 33 株大豆根瘤菌进行了基因型多样性研究。用固氮菌摄取氢酶探针 pHU52 对 15 株吸氢阴性菌株的 DNA 进行分析，结果表明 EcoRI 限制 DNA 的同源性和限制片段长度多态性存在差异。基于 4 种限制酶和固氮菌结瘤基因座 pRJUT10 的 DNA-DNA 杂交分析对 33 株菌进行聚类分析，表明存在 4 组菌株，它们之间的相似性小于 70%。用 BamHI 消化基因组 DNA 并用 pRJUT10 进行 DNA-DNA 杂交，可根据特定的指纹图谱对每种菌株进行分类。
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