来自嗜松头孢霉（Cephalosporium charticola Lindau）的糖化酶的分离及其某些性质
Isolation and Some Properties of Glucoamylase from Cephalosporium charticola Lindau.
作者信息
Krzechowska M, Urbanek H
机构信息
Laboratory of Enzymology, Institute of Physiology and Cytology, University of Lodz, 90-237 Lodz, Poland.
出版信息
Appl Microbiol. 1975 Aug;30(2):163-6. doi: 10.1128/am.30.2.163-166.1975.
High glucoamylase (alpha-D-/1 --> 4/glucan glucohydrolase, EC 3.2.1.3.) activity was obtained in the cell-free culture fluid of Cephalosporium charticola. Glucoamylase seems to be the only amylolytic enzyme produced by C. charticola. The enzyme, purified on diethylaminoethyl-cellulose, was homogeneous by disc gel electrophoresis. The optimum pH on starch was 5.4, and optimum temperature was 60 C. Starch was degraded more rapidly than several other substrates; maltose was hydrolyzed about one-fifth as rapidly as starch. The molecular weight was 69,000, as determined by Sephadex G-100 filtration. The enzyme is a glycoprotein and contains about 6.6% sugars (mannose and glucosamine).
在柱状头孢霉的无细胞培养液中获得了高活性的葡糖淀粉酶（α-D-1,4-葡聚糖葡糖水解酶，EC 3.2.1.3）。葡糖淀粉酶似乎是柱状头孢霉产生的唯一淀粉分解酶。该酶在二乙氨基乙基纤维素上纯化，经圆盘凝胶电泳显示为均一的。其作用于淀粉的最适pH为5.4，最适温度为60℃。淀粉比其他几种底物降解得更快；麦芽糖的水解速度约为淀粉的五分之一。通过葡聚糖G-100过滤测定，该酶的分子量为69,000。该酶是一种糖蛋白，含糖量约为6.6%（甘露糖和葡糖胺）。
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