人工细胞器：由模拟细胞的聚合物囊泡进行ATP合成。
Artificial organelle: ATP synthesis from cellular mimetic polymersomes.
作者信息
Choi Hyo-Jick, Montemagno Carlo D
机构信息
Department of Bioengineering, Room 7523 Boelter Hall, 420 Westwood Plaza, University of California-Los Angeles, PO Box 951600, Los Angeles, CA 90095-1600, USA.
出版信息
Nano Lett. 2005 Dec;5(12):2538-42. doi: 10.1021/nl051896e.
A complex cellular process was reconstructed using a multiprotein polymersome system. ATP has been produced by coupled reactions between bacteriorhodopsin, a light-driven transmembrane proton pump, and F(0)F(1)-ATP synthase motor protein, reconstituted in polymersomes. This indicates that ATP synthase maintained its ATP synthesis and therefore its motor activity in the artificial membranes. This hybrid proteopolymersome will have wide application in a number of fields ranging from the in vitro investigation of cellular metabolism to the synthesis of functional "smart" materials.
利用多蛋白聚合物囊泡系统重建了一个复杂的细胞过程。在聚合物囊泡中重组的细菌视紫红质（一种光驱动的跨膜质子泵）与F(0)F(1)-ATP合酶运动蛋白之间的偶联反应产生了ATP。这表明ATP合酶在人工膜中维持了其ATP合成以及运动活性。这种杂合蛋白聚合物囊泡将在从细胞代谢的体外研究到功能性“智能”材料合成等多个领域有广泛应用。
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