通过免疫组织化学染色和荧光原位杂交检测发现，儿童间变性大细胞淋巴瘤中ALK基因重排的发生率很高：一项遗传学与病理学的相关性研究。

Childhood anaplastic large cell lymphoma has a high incidence of ALK gene rearrangement as determined by immunohistochemical staining and fluorescent in situ hybridisation: a genetic and pathological correlation.

作者信息

Perkins Sherrie L, Pickering Diane, Lowe Eric J, Zwick David, Abromowitch Minnie, Davenport Ginny, Cairo Mitchell S, Sanger Warren G

机构信息

Department of Pathology, University of Utah Health Sciences and ARUP Institute, Salt Lake City, UT 84132, USA.

出版信息

Br J Haematol. 2005 Dec;131(5):624-7. doi: 10.1111/j.1365-2141.2005.05808.x.

DOI:10.1111/j.1365-2141.2005.05808.x

PMID:16351638

Abstract

Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.

摘要

间变性大细胞淋巴瘤（ALCL）占儿童非霍奇金淋巴瘤（NHL）的10%-15%。系统性ALCL与间变性淋巴瘤激酶（ALK）基因易位高度相关，伴有ALK蛋白过表达。我们采用荧光原位杂交（FISH）和ALK免疫组化染色对43例儿童系统性ALCL进行了ALK重排研究。FISH（对35例进行检测）在29例（83%）中检测到易位。免疫组化在42/43例（97%）中检测到ALK过表达，唯一ALK阴性的病例通过FISH显示有ALK重排，表明ALK易位发生率为100%。