用逆转录病毒转导分枝杆菌抗原85A基因的树突状细胞进行免疫接种可引发特异性细胞免疫，包括针对抗原85A上一个表位的细胞毒性T淋巴细胞活性。
Immunization with dendritic cells retrovirally transduced with mycobacterial antigen 85A gene elicits the specific cellular immunity including cytotoxic T-lymphocyte activity specific to an epitope on antigen 85A.
作者信息
Nakano Hideki, Nagata Toshi, Suda Takafumi, Tanaka Takao, Aoshi Taiki, Uchijima Masato, Kuwayama Sachiko, Kanamaru Noriko, Chida Kingo, Nakamura Hirotoshi, Okada Masaji, Koide Yukio
机构信息
Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handa-yama, Hamamatsu 431-3192, and Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, Osaka, Japan.
出版信息
Vaccine. 2006 Mar 15;24(12):2110-9. doi: 10.1016/j.vaccine.2005.11.026. Epub 2005 Nov 28.
In the present study, we evaluated antigen 85A (Ag85A) gene-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A gene and injected to BALB/c mice intravenously. The DC vaccine was capable of inducing purified protein derivative (PPD)- and the antigen-specific spleen cell proliferation and IFN-gamma production from both CD4+ and CD8+ T cells in spleens of the immune mice. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) and IFN-gamma-producing cells specific for a 9-mer CTL epitope on Ag85A molecule. This eliciting cellular immunity led to protection against wasting disease due to M. tuberculosis infection and induction of moderate bacterial clearance.
在本研究中，我们评估了抗原85A（Ag85A）基因转导的树突状细胞（DCs）疫苗对结核分枝杆菌的作用。用分枝杆菌Ag85A基因通过逆转录病毒转导小鼠骨髓来源的DCs，并静脉注射到BALB/c小鼠体内。该DC疫苗能够诱导免疫小鼠脾脏中纯化蛋白衍生物（PPD）和抗原特异性脾细胞增殖以及CD4+和CD8+T细胞产生γ干扰素。此外，DC疫苗接种诱导了针对Ag85A分子上一个9聚体CTL表位的细胞毒性T淋巴细胞（CTL）和产生γ干扰素的细胞。这种引发的细胞免疫导致对结核分枝杆菌感染引起的消瘦病的保护作用，并诱导适度的细菌清除。
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