一种用于小体积禽血清中有机氯农药和多氯联苯的简单快速提取方法。
A simple and fast extraction method for organochlorine pesticides and polychlorinated biphenyls in small volumes of avian serum.
作者信息
Sundberg Sarah E, Ellington J Jackson, Evans John J
机构信息
National Exposure Research Laboratory, U.S. Environmental Protection Agency, 960 College Station Road, Athens GA 30605, USA.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):99-104. doi: 10.1016/j.jchromb.2005.11.037. Epub 2005 Dec 13.
A solid-phase extraction (SPE) method was developed using 8M urea to desorb and extract organochlorine pesticides (OCs) and polychlorinated biphenyls (PCBs) from avian serum for analysis by capillary gas chromatography with electron capture detection (GC-ECD). The analytes were efficiently extracted from the denatured serum-lipoprotein-analyte complex by one passage through an Oasis((R)) hydrophilic-lipophilic-balanced (HLB) SPE cartridge. No further clean-up was necessary, the entire extraction procedure and GC-ECD analysis can be accomplished in less than 3h. Serum volumes ranged from 100 microL to 1 mL with absolute recoveries of 90-101% for PCBs and 74% to 101% for the OC pesticides.
开发了一种固相萃取（SPE）方法，该方法使用8M尿素从禽血清中解吸并萃取有机氯农药（OCs）和多氯联苯（PCBs），以通过带电子捕获检测的毛细管气相色谱法（GC-ECD）进行分析。通过使变性的血清-脂蛋白-分析物复合物通过一个Oasis®亲水亲脂平衡（HLB）SPE小柱一次，可有效地从其中萃取分析物。无需进一步净化，整个萃取过程和GC-ECD分析可在不到3小时内完成。血清体积范围为100微升至1毫升，PCBs的绝对回收率为90-101%，OC农药的绝对回收率为74%-101%。
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