Izvol'skaia M S, Adamskaia E I, Voronova S N, Duittoz A, Tillet I
Ontogenez. 2005 Nov-Dec;36(6):440-8.
The contents of dopamine, serotonin, and noradrenaline in rat fetuses developing under the conditions of their deficiency induced by administration of alpha-methyl-para-tyrosine to females during 11th to 16th or 20th day of pregnancy and in fetuses, whose mothers were given saline at the same time, were determined using HPLC with subsequent electrochemical detection. Administration of alpha-methyl-para-tyrosine led to decreased levels of dopamine and noradrenaline in the areas of migration of GnRH-neurons in fetuses on days 17 and 21 of prenatal development. The concentration of serotonin remained unchanged, except in the head nasal area in males on day 21. The areas of interaction between the brain catecholaminergic systems and migrating and differentiating GnRH-neurons were determined by double immunohistochemical labeling. Close topographical location of GnRH-immunoreactive neurons and tyrosine hydroxylase-immunoreactive in the area of nucleus accumbens on days 17 and 20, as well as in the median eminence on day 20. The GnRH concentration in the caudal areas of migration of GnRH-neurons under the normal conditions and in the case of catecholamine deficiency was determined using radioimmunoassay. After administration of alpha-methyl-para-tyrosine the GnRH concentration in the anterior hypothalamus decreased in females. The data obtained suggest the involvement of catecholamines in the regulation of development of GnRH-Neurons during prenatal development. In addition, the adequacy and efficiency of the used model of catecholamine deficiency for studying the development of such neurons was confirmed.
在孕期第11至16天或第20天给雌性大鼠注射α-甲基-对-酪氨酸，诱导其多巴胺、5-羟色胺和去甲肾上腺素缺乏，以此为条件发育的大鼠胎儿以及同时给其母亲注射生理盐水的胎儿，采用高效液相色谱法并结合后续电化学检测，测定了其体内多巴胺、5-羟色胺和去甲肾上腺素的含量。在产前发育的第17天和第21天，注射α-甲基-对-酪氨酸导致胎儿GnRH神经元迁移区域内的多巴胺和去甲肾上腺素水平降低。5-羟色胺浓度保持不变，但在第21天雄性胎儿的头部鼻区除外。通过双重免疫组织化学标记确定了脑内儿茶酚胺能系统与迁移和分化的GnRH神经元之间的相互作用区域。在第17天和第20天，伏隔核区域以及第20天正中隆起处，GnRH免疫反应性神经元和酪氨酸羟化酶免疫反应性神经元在地形上位置相近。采用放射免疫分析法测定了正常条件下以及儿茶酚胺缺乏情况下GnRH神经元尾侧迁移区域内的GnRH浓度。给雌性大鼠注射α-甲基-对-酪氨酸后，下丘脑前部的GnRH浓度降低。所获数据表明，儿茶酚胺参与产前发育期间GnRH神经元发育的调节。此外，还证实了所采用的儿茶酚胺缺乏模型对于研究此类神经元发育的充分性和有效性。
