在大鼠栓塞性中风模型中，溶栓可减少梗死体积并降低死亡率。

Reduction of infarct volume and mortality by thrombolysis in a rat embolic stroke model.

作者信息

Overgaard K, Sereghy T, Boysen G, Pedersen H, Diemer N H

机构信息

Department of Neurology, University Hospital of Copenhagen, Denmark.

出版信息

Stroke. 1992 Aug;23(8):1167-73; discussion 1174. doi: 10.1161/01.str.23.8.1167.

DOI:10.1161/01.str.23.8.1167

PMID:1636193

Abstract

BACKGROUND AND PURPOSE

Thrombolytic therapy with recombinant tissue plasminogen activator was tested in a rat embolic stroke model.

METHODS

The rat carotid territory was embolized with arterial-like microthrombi formed under pressure. Hemispheric cerebral blood flow before and after embolization was measured by the intraarterial Xenon-133 injection method. Fifteen minutes after embolization, 24 rats were treated with 3 mg/kg or 10 mg/kg tissue plasminogen activator, and 27 were treated with saline. Carotid angiography displayed the rate of occlusion of the cerebral arterial supply before and after treatment. Brains were fixed and evaluated neuropathologically and infarct volume was measured.

RESULTS

Cerebral blood flow was reduced 70-86% after embolization. The comparison of pretreatment and posttreatment angiography showed significant (p = 0.0005) reperfusion in the treated rats. Thrombolytic therapy significantly reduced the infarct volume from 55.1% to 24.4% of embolized hemisphere volume (p = 0.007) and increased the survival rate from 0.48 to 0.96 (p = 0.0004). Fifty-three percent of the embolized rats recanalized completely after thrombolytic treatment and developed almost no infarction (median volume 2.8%), and all survived. No hemorrhagic complications were observed.

CONCLUSIONS

Early thrombolytic therapy induced recanalization and reduced mortality and infarct volume after embolic stroke in this model.

摘要

背景与目的

在大鼠栓塞性脑卒中模型中对重组组织型纤溶酶原激活剂溶栓治疗进行了测试。

方法

用压力下形成的类动脉微血栓栓塞大鼠颈动脉区域。通过动脉内注射氙 - 133 法测量栓塞前后的半球脑血流量。栓塞后 15 分钟，24 只大鼠接受 3mg/kg 或 10mg/kg 组织型纤溶酶原激活剂治疗，27 只大鼠接受生理盐水治疗。颈动脉血管造影显示治疗前后脑动脉供血的闭塞率。将大脑固定并进行神经病理学评估，测量梗死体积。

结果

栓塞后脑血流量减少 70 - 86%。治疗前后血管造影的比较显示，治疗组大鼠有显著（p = 0.0005）再灌注。溶栓治疗使梗死体积从栓塞半球体积的 55.1%显著减少至 24.4%（p = 0.007），并使生存率从 0.48 提高至 0.96（p = 0.0004）。53%的栓塞大鼠在溶栓治疗后完全再通，几乎未发生梗死（中位体积 2.8%），且全部存活。未观察到出血并发症。